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G Protein-Coupled Receptor Signaling in Plants

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Mark P. Running

    1. Department of Biology, University of Louisville, Louisville, USA

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  • Explores techniques to examine the roles of GCRPs and small GTPases in key plant biological processes
  • Features step-by-step protocols that are ready for the lab
  • Includes expert tips and key implementation advice to ensure quality results

Part of the book series: Methods in Molecular Biology (MIMB, volume 1043)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Topology Assessment, G Protein-Coupled Receptor (GPCR) Prediction, and In Vivo Interaction Assays to Identify Plant Candidate GPCRs

    • Timothy E. Gookin, Jannick D. Bendtsen
    Pages 1-12

  3. Measurement of GTP-Binding and GTPase Activity of Heterotrimeric Gα Proteins

    • Swarup Roy Choudhury, Corey S. Westfall, Dieter Hackenberg, Sona Pandey
    Pages 13-20

  4. Biochemical Analysis of the Interaction Between Phospholipase Dα1 and GTP-Binding Protein α-Subunit from Arabidopsis thaliana

    • Jian Zhao, Xuemin Wang
    Pages 21-35

  5. Analysis of Cell Division and Cell Elongation in the Hypocotyls of Arabidopsis Heterotrimeric G Protein Mutants

    • Zhaoqing Jin, Wellington Muchero, Jin-Gui Chen
    Pages 37-43

  6. Aequorin Luminescence-Based Functional Calcium Assay for Heterotrimeric G-Proteins in Arabidopsis

    • Kiwamu Tanaka, Jeongmin Choi, Gary Stacey
    Pages 45-54

  7. Methods for Analysis of Disease Resistance and the Defense Response in Arabidopsis

    • Guojing Li, Xiujuan Zhang, Dongli Wan, Shuqun Zhang, Yiji Xia
    Pages 55-66

  8. Fusarium oxysporum Infection Assays in Arabidopsis

    • Yuri Trusov, David Chakravorty, Jose Ramon Botella
    Pages 67-72

  9. Analysis of Unfolded Protein Response in Arabidopsis

    • Yani Chen, Federica Brandizzi
    Pages 73-80

  10. Functional Analysis of Heterotrimeric G Proteins in Chloroplast Development in Arabidopsis

    • Wenjuan Wu, Jirong Huang
    Pages 81-87

  11. G Protein Signaling in UV Protection: Methods for Understanding the Signals in Young Etiolated Seedlings

    • Danielle A. Orozco-Nunnelly, Lon S. Kaufman, Katherine M. Warpeha
    Pages 89-101

  12. Functional Analysis of Small Rab GTPases in Cytokinesis in Arabidopsis thaliana

    • Xingyun Qi, Huanquan Zheng
    Pages 103-112

  13. In Vivo Localization in Arabidopsis Protoplasts and Root Tissue

    • Myoung Hui Lee, Yongjik Lee, Inhwan Hwang
    Pages 113-120

  14. Analysis of Protein Prenylation and S-Acylation Using Gas Chromatography–Coupled Mass Spectrometry

    • Nadav Sorek, Amir Akerman, Shaul Yalovsky
    Pages 121-134

  15. In Vitro Myristoylation Assay of Arabidopsis Proteins

    • Xuehui Feng, Wan Shi, Xuejun Wang, Mark P. Running
    Pages 135-139

  16. Assaying Protein S-Acylation in Plants

    • Piers A. Hemsley
    Pages 141-146

  17. In Vitro Prenylation Assay of Arabidopsis Proteins

    • Wan Shi, Qin Zeng, Mark P. Running
    Pages 147-160

  18. Back Matter

    Pages 161-162
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Keywords

About this book

Remarkably, while G protein-coupled receptors (GPCRs) are highly prevalent in animals and yeast, very few candidate GPCRs have been identified in plants. In G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols, experts in the field describe techniques used in the study of small GTPases and related proteins.  Beginning with a chapter on bioinformatics approaches for GPCR discovery, this detailed volume continues with chapters on heterotrimeric G protein subunits, Rab-GTPases, as well as lipid modifications, including myristoylation, acylation, and prenylation.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Practical and dependable, G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols aims to aid further studies into the roles of small GTPases which will help elucidate numerous key processes in plants.

Editors and Affiliations

  • Department of Biology, University of Louisville, Louisville, USA

    Mark P. Running

Bibliographic Information

  • Book Title: G Protein-Coupled Receptor Signaling in Plants

  • Book Subtitle: Methods and Protocols

  • Editors: Mark P. Running

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-532-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-531-6Published: 05 August 2013

  • Softcover ISBN: 978-1-4939-6216-7Published: 23 August 2016

  • eBook ISBN: 978-1-62703-532-3Published: 04 August 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 162

  • Number of Illustrations: 5 b/w illustrations, 14 illustrations in colour

  • Topics: Plant Sciences, Proteomics

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