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Implementation of HEA at Blood Centers: Prescreening, Rare Donors, Inventory Management

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BeadChip Molecular Immunohematology

Abstract

The BeadChip™ system, one of the commercial platforms currently available for predicting human erythrocyte antigen (HEA) phenotypes by DNA analysis, has shown utility in blood bank operations and hospital transfusion services since its introduction in 2006. This chapter compiles information on the implementation and performance of extended HEA typing using the BeadChip™ platform in US-based blood centers and hospital transfusion services. LifeShare Blood Centers, Gulf Coast Regional Blood Center, LifeSouth Community Blood Center, and the New York Blood Center represent some of the nation’s largest independent blood centers and together collect over 1.5 million blood units annually. The combined experience of these centers ­provides an overview of the impact of HEA BeadChip™ implementation on the provision of compatible blood for patients, on complex patient work ups, and also on blood bank operation by discussing the process flow, concordance, and economic impact.

BeadChip User Group at Blood Centers: Katrina Billingsley (LifeShare Blood Centers, LA), Cheri Jennings (Gulf Coast Regional Blood Center, TX), Carla Collins (Gulf Coast Regional Blood Center, TX), Cindy Sapp (Gulf Coast Regional Blood Center, TX), Donna Strauss (New York Blood Center, NY), Kimberly Nail (Central Illinois Community Blood Center, IL), Bill Martinez (LifeSouth Blood Center, FL), Dennis Warner (LifeSouth Blood Center, FL).

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References

  1. DHHS (2007) The 2007 National Blood Collection and Utilization Survey Report. http://www.hhs.gov/ophs/bloodsafety/2007nbcus_survey.pdf. Accessed 21 Oct 2008

  2. AHRQ (2009) Blood transfusions more than double since 1997. AHRQ News and Numbers. http://www.ahrq.gov/news/nn/nn092409.htm. Accessed 24 Sep 2009

  3. Daniels G, Castilho L, Flegel WA et al. (2009) International Society of Blood Transfusion Committee on terminology for red blood cell surface antigens: Macao report. Vox Sang 96:153–156

    Article  CAS  PubMed  Google Scholar 

  4. Poole J, Daniels G (2007) Blood group antibodies and their significance in transfusion medicine. Transfus Med Rev 21:58–71

    Article  PubMed  Google Scholar 

  5. Higgins JM, Sloan SR (2008) Stochastic modeling of human RBC alloimmunization: evidence for a distinct population of immunologic responders. Blood 112:2546–2553

    Article  CAS  PubMed  Google Scholar 

  6. Tatari-Calderone Z, Minniti CP, Kratovil T et al. (2009) rs660 polymorphism in Ro52 (SSA1;TRIM) is a marker for age-dependent tolerance induction and efficiency of alloimmunization in sickle cell disease. Mol Immunol 47:64–70

    Article  CAS  PubMed  Google Scholar 

  7. Hendrickson JE, Chadwick TE, Roback JD et al. (2007) Inflammation enhances consumption and presentation of transfused RBC antigens by dendritic cells. Blood 110:2736–2743

    Article  CAS  PubMed  Google Scholar 

  8. FDA (2009) Fatalities reported to FDA following blood collection and transfusion. Annual summary for fiscal year 2009. http://www.fda.gov/cber/bloodhttp://www.fda.gov/downloads/BiologicsBloodVaccines/SafetyAvailability/ReportaProblem/TransfusionDonationFatalities/UCM205620.pdf

  9. SHOT (2009) SHOT report 2008 http://www.shotuk.org/wp-content/uploads/2010/03/SHOT-Report-2008.pdf. Accessed 27 Aug 2009

  10. Engelfriet CP, Reesink HW, Fontão-Wendel R et al. (2006) Prevention and diagnosis of delayed haemolytic transfusion reactions. Vox Sang 91:353–368

    CAS  PubMed  Google Scholar 

  11. Vamvakas EC, Blajchman MA (2010) Blood still kills: six strategies to further reduce allogeneic blood transfusion-related mortality. Transfus Med Rev 24:77–124

    Article  PubMed  Google Scholar 

  12. Strauss D, Reid ME (2008) Value of DNA-based assays for donor screening and regulatory issues. Immunohematology 24:175–179

    CAS  PubMed  Google Scholar 

  13. Beiboer SH, Wieringa-Jelsma T, Maaskant-Van Wijk PA, van der Schoot CE, van Zwieten R, Roos D, den Dunnen JT, de Haas M (2005) Rapid genotyping of blood group antigens by multiplex polymerase chain reaction and DNA microarray hybridization. Transfusion 45(5):667–679

    Google Scholar 

  14. Bugert P, McBride S, Smith G, Dugrillon A, Klütter H, Ouwehand WH, Metcalfe P (2005) Microarray-based genotyping for blood groups: comparison of gene array and 5¢-nuclease assay techniques with human platelet antigen as a model. Transfusion 45(5):654–659

    Google Scholar 

  15. Denomme GA, Van Oene M (2005) High-throughput multiplex single-nucleotide polymorphism analysis for red cell and platelet antigen genotypes. 45(5):660–666

    Google Scholar 

  16. Hashmi G, Shariff T, Seul M et al. (2005) A flexible array format for large-scale, rapid blood group DNA typing. Transfusion 45(5):680–688

    Article  CAS  PubMed  Google Scholar 

  17. Westhoff CM (2006) Molecular testing for transfusion medicine. Current Opinions in Hematology 13(6):471–475

    Google Scholar 

  18. Avent ND, Martinez A, Flegel WA, et al. (2007) The BloodGen project: toward mass-scale comprehensive genotyping of blood donors in the European Union and beyond. Transfusion 47(1 Suppl):40s–46s

    Google Scholar 

  19. Hashmi G, Shariff T, Zhang Y et al. (2007) Determination of 24 minor red blood cell antigens for more than 2000 blood donors by high-throughput DNA analysis. Transfusion 47:736–747

    Article  CAS  PubMed  Google Scholar 

  20. Hashmi G (2007) Red blood cell antigen phenotype by DNA analysis. Transfusion 47(1 Suppl):60S–63S

    Article  CAS  PubMed  Google Scholar 

  21. Ribeiro KR, Guarnieri MH, da Costa DC et al. (2009) DNA array analysis for red blood cell antigens facilitates the transfusion support with antigen-matched blood in patients with sickle cell disease. Vox Sang 97:147–152

    Article  CAS  PubMed  Google Scholar 

  22. Klapper E, Zhang Y, Figueroa P, et al. (2010) Toward extended phenotype matching: a new operational paradigm for the transfusion service, Transfusion 50(3):536–546

    Google Scholar 

  23. American Rare Donor Program (2010) Version 2-1-2010. Philadelphia, PA

    Google Scholar 

  24. Zhang Y, Hashmi G, Seul M et al. (2008) Random selection of donor units for extended ­phenotype determination and efficient allocation to prospective recipients. Transfusion 48(Suppl):206A

    Google Scholar 

  25. Wun T, Hassell K (2009) Best practices for transfusion for sickle cell disease patients. Hematol Rev 1:e22

    Google Scholar 

  26. Centers for Medicare and Medicaid Services: Program Descriptions/Projects (2010). http://www.cms.gov/CLIA/07_Program_Descriptions_Projects.asp. Accessed 16 Jun 2010

  27. Moulds JM, Billingsley KL, Moulds JJ (2009) Results of a proficiency testing program for microarray users. Transfusion 49(Suppl):137A (SP226)

    Google Scholar 

  28. Allen T, Billingsley KL, Slaughter J et al. (2009) Red cell genotyping: a cost effective approach to screening large numbers of donors. Transfusion 49(Suppl):135A (SP220)

    Google Scholar 

  29. AABB (2008) Standards for molecular testing of red cell, platelet, and neutrophil antigens, 1st edn. AABB, Bethesda

    Google Scholar 

  30. AABB (2009) Guidance for standards for molecular testing of red cell, platelet, and neutrophil antigens, 1st edn. AABB, Bethesda

    Google Scholar 

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Acknowledgments

We acknowledge Carolyn Whitsett, MD, for her critical review of the manuscript. We also recognize BeadChip users for their contributions, and Ermelina Enriquez, BS, at BioArray’s mih laboratory, and members of the technical marketing team, Ruth Huang, BS, Kevin Trainer, BS, and Tasmia Shariff, BS, for data compilation.

Author information

Authors and Affiliations

  1. For the BeadChip user group at Blood Centers and LifeShare Blood Centers, Shreveport, LA, USA

    JoAnn M. Moulds

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  1. JoAnn M. Moulds
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Correspondence to JoAnn M. Moulds .

Editor information

Editors and Affiliations

  1. Transfusion Medicine Div., Johns Hopkins Hospital, N. Wolfe St. 600, Baltimore, 21287-6667, Maryland, USA

    Paul M. Ness

  2. Pediatric Transfusion Medicine, Children's Hospital Boston, Longwood Avenue 300, Boston, 02115, Massachusetts, USA

    Steve R. Sloan

  3. Scientific Support Services, LifeShare Blood Centers, Linwood Avenue 8910, Shreveport, 71106, Louisiana, USA

    JoAnn M. Moulds

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Moulds, J.M. (2011). Implementation of HEA at Blood Centers: Prescreening, Rare Donors, Inventory Management. In: Ness, P., Sloan, S., Moulds, J. (eds) BeadChip Molecular Immunohematology. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-7512-6_4

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