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Fluorescent Protein FRET Applications

Protein Engineering, Intracellular Sensing, and Interaction Screening

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Part of the book series: Reviews in Fluorescence 2008 ((RFLU,volume 2008))

Abstract

Fluorescent proteins provide the opportunity to glimpse inside a living cell and obtain useful biochemical information pertaining to a variety of biological systems. These probes are unique when compared with synthetic fluorophores since they are genetically encoded and can be expressed and retained within the living cell. And, importantly, fluorescent protein fusions have enabled investigation of intracellular transport and localization of various proteins [1–3]. While fluorescent proteins are already ubiquitous in biological research, they are set to become key tools for intracellular sensing, reversible protein interaction screening, and protein engineering.

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Correspondence to Patrick S. Daugherty .

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Nguyen, A.W., You, X., Jabaiah, A.M., Daugherty, P.S. (2010). Fluorescent Protein FRET Applications. In: Geddes, C.D. (eds) Reviews in Fluorescence 2008. Reviews in Fluorescence 2008, vol 2008. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-1260-2_13

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