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A Practical Guide to Frozen Section Technique pp 117–129Cite as

Fixation, Staining and Coverslipping of Frozen Section Slides

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Abstract

This chapter covers fixation, staining and coverslipping of frozen section slides. A number of fixatives and fixative cocktails in use are described and illustrated as well as artifacts resulting from prolonged drying. Several commonly used staining procedures are described with suggestions on maximizing the quality and information gathered for interpretation. Adhesion of tissue to glass slides and causes of poor adhesion is discussed. Problems arising in the coverslipping process are discussed and method for coverslipping finished slides is offered.

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References

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Authors and Affiliations

  1. Department of Pathology, University of Medicine and Dentistry of New Jersey Pathology Innovations, LLC, New Jersey, NJ, 07481, USA

    Stephen R. Peters

Authors
  1. Stephen R. Peters
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  2. Catherine Susan Delia
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Corresponding author

Correspondence to Stephen R. Peters .

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Editors and Affiliations

  1. Old Mill Lane 410, Wyckoff, 07481, USA

    Stephen R. Peters

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© 2010 Springer Science+Business Media, LLC

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Peters, S.R., Delia, C.S. (2010). Fixation, Staining and Coverslipping of Frozen Section Slides. In: Peters, S. (eds) A Practical Guide to Frozen Section Technique. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-1234-3_6

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  • DOI: https://doi.org/10.1007/978-1-4419-1234-3_6

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4419-1233-6

  • Online ISBN: 978-1-4419-1234-3

  • eBook Packages: MedicineMedicine (R0)

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