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EPR Studies of the Primary Electron Donor P700 in Photosystem

  • Chapter
Photosystem I

Part of the book series: Advances in Photosynthesis and Respiration ((AIPH,volume 24))

Abstract

The primary donor P700 in Photosystem I (PS I) is a heterodimer comprised of a chlorophyll a and a chlorophyll á. The electronic structure of this species, which is related to its function in vivo, can be studied by EPR techniques applied to the paramagnetic states P700+ (cation radical) and 3P700 (triplet state) of the primary donor. In the case of P700+ observables are the electronic g tensor and the electron-nuclear hyperfine and nuclear quadrupole coupling tensors; in the case of 3P700 the electron–electron dipolar coupling tensor serves as an additional probe. In this contribution, the determination of the magnetic resonance parameters by EPR techniques are described. Conclusions about the electronic structure, in particular about the spin and charge density distribution in this species, are drawn. The results are corroborated by studies of model systems and of the primary donor in genetically modified photosystem I preparations, which gives information on the effect of the protein surroundings. Emphasis is placed on a theoretical description of P700 in its various states, which is based on a comparison with molecular orbital calculations. Implications of the experimental findings for the functional properties of the primary donor in photosystem I are discussed.

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  1. Max-Planck-Institut fÜr Bioanorganische Chemie, Stiftstraße 34-36, MÜlheim an der Ruhr, D-45470, Germany

    Wolfgang Lubitz

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  1. The Pennsylvania State University, Pennsylvania, USA

    John H. Golbeck

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Lubitz, W. (2006). EPR Studies of the Primary Electron Donor P700 in Photosystem. In: Golbeck, J.H. (eds) Photosystem I. Advances in Photosynthesis and Respiration, vol 24. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-4256-0_17

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