Vascular smooth muscle cells (SMCs) synthesise collagens type I and V matrix proteins, which are major constituents of the arterial wall. In culture, matrix gene expression varies inversely with the rate of SMC proliferation. Previously we showed that B-myb, a member of the myb gene family, is expressed in SMCs in a cell-cycle dependent fashion, and that it is a negative regulator of matrix gene transcription. Phosphorylation by cyclin A/cdk2 relieved B-Myb-mediated repression of α2 (V) collagen gene transcription, and the sites of phosphorylation were distinct from those affecting activation by B-Myb. The domain responsible for repression mapped to residues 491 to 582 of the C-terminal region of B-Myb. Transgenic mice over-expressing BMyb displayed significantly reduced collagen expression in the aorta. Thus, B-Myb functions in vivo as a repressor of collagen gene expression in vascular SMCs.
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© 2004 Kluwer Academic Publishers
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Hofmann, C.S., Sonenshein, G.E. (2004). Repression Of Matrix Gene Expression By B-Myb. In: Frampton, J. (eds) Myb Transcription Factors: Their Role in Growth, Differentiation and Disease. Proteins and Cell Regulation, vol 2. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-2869-4_18
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DOI: https://doi.org/10.1007/978-1-4020-2869-4_18
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