Abstract
Grasses of the African genus Brachiaria are the most widely planted forages in Brazil. We previously reported the construction of microsatellite-enriched libraries for five Brachiaria species. Now the development of microsatellite markers for B. brizantha and their use for the genetic characterization of morphologically divergent accessions of the germplasm collection of Embrapa are presented. Fifteen pairs of primers were designed and assayed on 23 genotypes. From the fifteen loci evaluated, ten were polymorphic. The divergence between genotypes was estimated using Jaccard's coefficient of similarity and UPGMA method was used for clustering genotypes. The results showed that microsatellites are powerful tools for characterizing genetic diversity of Brachiaria species.
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Jungmann, L., Francisco, P., Sousa, A., Paiva, J., do Valle, C., de Souza, A. (2009). Development of Microsatellite Markers for Brachiaria brizantha and Germplasm Diversity Characterization of this Tropical Forage Grass. In: Molecular Breeding of Forage and Turf. Springer, New York, NY. https://doi.org/10.1007/978-0-387-79144-9_9
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DOI: https://doi.org/10.1007/978-0-387-79144-9_9
Publisher Name: Springer, New York, NY
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