Skip to main content
SpringerLink
Log in

DREB Regulons in Abiotic-Stress-Responsive Gene Expression in Plants

  • Conference paper
Molecular Breeding of Forage and Turf

Abstract

Plant growth and productivity is affected by various abiotic stresses such as drought, high salinity, and low temperature. Expression of a variety of genes is induced by these stresses in various plants. In the signal transduction network from perception of stress signals to stress-responsive gene expression, various transcription factors and cis-acting elements in the stress-responsive gene expression function for plant adaptation to environmental stresses. The dehydration-responsive element (DRE)/C-repeat (CRT) cis-acting element is involved in osmotic- and cold-stress-inducible gene expression. Transcription factors that bind to the DRE/CRT were isolated and named DREB1/CBF and DREB2. DREB1/CBF regulon is involved in cold-stress-responsive gene expression, whereas, DREB2 is involved in osmotic-stress-responsive gene expression. Recently, we highlight transcriptional regulation of gene expression in response to drought and cold stresses, with particular emphasis on the role of DREB regulon in stress-responsive gene expression.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bray E, Bailey-Serres J, Weretilnyk E (2000) Responses to abiotic stresses. In: Buchanan BB, Gruissem W, Jones RL (eds) Biochemistry and Molecular Biology of Plants, American Society of Plant Physiologists, Rockvillepp 1158–1203

    Google Scholar 

  • Cook D, Fowler S, Fiehn O, Thomashow MF (2004) A prominent role for the CBF cold response pathway in configuring the low-temperature metabolome of Arabidopsis. Proc Natl Acad Sci U S A 101: 15243–15248

    Article  PubMed  CAS  Google Scholar 

  • Cushman JC, Bohner HJ (2000) Genome approaches to plant stress tolerance. Curr Opin Plant Biol 3: 117–124

    Article  PubMed  CAS  Google Scholar 

  • Dubouzet JG, Sakuma Y, Ito Y, Kasuga M, Dubouzet EG, Miura S, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2003) OsDREB genes in rice, Oryza sativa. L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression Plant J 33: 751–763

    Article  PubMed  CAS  Google Scholar 

  • Fowler S, Thomashow MF (2002) Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway. Plant Cell 14: 1675–1690

    Article  PubMed  CAS  Google Scholar 

  • Gilmour SJ, Zarka DG, Stockinger EJ, Salazar MP, Houghton JM, Thomashow MF (1998) Low temperature regulation of Arabidopsis. CBF family of AP2 transcriptional activators as an early step in cold-induced COR gene expression Plant J 16: 433–442

    Article  PubMed  CAS  Google Scholar 

  • Gilmour SJ, Sebolt AM, Salazar MP, Everard JD, Thomashow MF (2000) Overexpression of the Arabidopsis CBF3 transcriptional activator mimics multiple biochemical changes associated with cold acclimation. Plant Physiol 124: 1854–1865

    Article  PubMed  CAS  Google Scholar 

  • Haake V, Cook D, Riechmann JL, Pineda O, Thomashow MF, Zhang JZ (2002) Transcription factor CBF4 is a regulator of drought adaptation in Arabidopsis. Plant Physiol 130: 639–648

    Article  PubMed  CAS  Google Scholar 

  • Holmberg N, Bulow L (1998) Improving stress tolerance in plants by gene transfer. Trends Plant Sci 3: 61–66

    Article  Google Scholar 

  • Hsieh TH, Lee JT, Yang PT, Chiu LH, Charng YY, Wang YC, Chan MT (2002a) Heterology expression of the Arabidopsis C-repeat/dehydration response element binding factor 1 gene confers elevated tolerance to chilling and oxidative stresses in transgenic tomato. Plant Physiol 129: 1086–1094

    Article  CAS  Google Scholar 

  • Hsieh TH, Lee JT, Charng YY, Chan MT (2002b) Tomato plants ectopically expressing Arabidopsis CBF1 show enhanced resistance to water deficit stress. Plant Physiol 130: 618–626

    Article  CAS  Google Scholar 

  • Ito Y, Katsura K, Maruyama K, Taji T, Kobayashi M, Shinozaki K, Yamaguchi-Shinozak K (2006) Functional analysis of rice DREB1/CBF-type transcription factors involved in cold-responsive gene expression in transgenic rice. Plant Cell Physiol 47: 141–153

    Article  PubMed  CAS  Google Scholar 

  • Jaglo KR, Kleff S, Amundsen KL, Zhang X, Haake V, Zhang JZ, Deits T, Thomashow MF (2001) Components of the Arabidopsis C-repeat/dehydration-responsive element binding factor cold-response pathway are conserved in Brassica napus. and other plant species Plant Physiol 127: 910–917

    Article  PubMed  CAS  Google Scholar 

  • Jaglo-Ottosen KR, Gilmour SJ, Zarka DG, Schabenberger O, Thomashow MF (1998) Arabidopsis. CBF1 overexpression induces cor genes and enhances freezing tolerance Science 280: 104–106

    Article  PubMed  CAS  Google Scholar 

  • Jiang C, Iu B, Singh J (1996) Requirement of a CCGAC cis. -acting element for cold induction of the BN115 gene from winter Brassica napus Plant Mol Biol 30: 679–684

    Article  PubMed  CAS  Google Scholar 

  • Kasuga M, Liu Q, Miura S, Yamaguchi-Shinozaki K, Shinozaki K (1999) Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor. Nat Biotechnol 17: 287–291

    Article  PubMed  CAS  Google Scholar 

  • Kasuga M, Miura S, Shinozaki K, Yamaguchi-Shinozaki K (2004) A combination of the Arabidopsis DREB1A. gene and stress-inducible rd29A promoter improved drought- and low-temperature stress tolerance in tobacco by gene transfer Plant Cell Physiol 45: 346–350

    Article  PubMed  CAS  Google Scholar 

  • Kreps JA, Wu Y, Chang HS, Zhu T, Wang X, Harper JF (2002) Transcriptome changes for Arabidopsis in response to salt, osmotic, and cold stress. Plant Physiol 130: 2129–2141

    Article  PubMed  CAS  Google Scholar 

  • Lim CJ, Hwang JE, Chen H, Hong JK, Yang KA, Choi MS, Lee KO, Chun WS, Lee SY, Lim CO (2007) Over-expression of the Arabidopsis DRE/CRT-binding transcription factor DREB2C enhances thermotolerance. Biochem Biophys Res Commun 362: 431–436

    Article  PubMed  CAS  Google Scholar 

  • Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S, Goda H, Shimada Y, Yoshida S, Shinozaki K, Yamaguchi-Shinozaki K (1998) Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell 10: 391–406

    Google Scholar 

  • Maruyama K, Sakuma Y, Kasuga M, Ito Y, Seki M, Goda H, Shimada Y, Yoshida S, Shinozaki K, Yamaguchi-Shinozaki K (2004) Identification of cold-inducible downstream genes of the Arabidopsis. DREB1A/CBF3 transcriptional factor using two microarray systems Plant J 38: 982–993

    Article  PubMed  CAS  Google Scholar 

  • Nakashima K, Shinwar ZK, Sakuma Y, Seki M, Miura S, Shinozaki K, Yamaguchi-Shinozaki K (2000) Organization and expression of two Arabidopsis DREB2. genes encoding DRE-binding proteins involved in dehydration- and high-salinity-responsive gene expression Plant Mol Biol 42: 657–665

    Article  PubMed  CAS  Google Scholar 

  • Oh SJ, Song SI, Kim YS, Jang H, Kim SY, Kim M, Kim YK, Nahm BH, Kim, JK (2005) Arabidopsis CBF3/DREB1A and ABF3 in transgenic rice increased tolerance to abiotic stress without stunting growth. Plant Physiol 138: 341–351

    Article  PubMed  CAS  Google Scholar 

  • Ouellet F, Vazquez-Tello A, Sarhan F (1998) The wheat wcs120 promoter is cold-inducible in both monocotyledonous and dicotyledonous species. FEBS Lett 423: 324–328

    Article  PubMed  CAS  Google Scholar 

  • Pellegrineschi A, Reynolds M, Pacheco M, Brito RM, Almeraya R, Yamaguchi-Shinozaki K, Hoisington D (2004) Stress-induced expression in wheat of the Arabidopsis thaliana DREB1A. gene delays water stress symptoms under greenhouse conditions Genome 47: 493–500

    Article  PubMed  CAS  Google Scholar 

  • Qin F, Sakuma Y, Li J, Liu Q, Li YQ, Shinozaki K, Yamaguchi-Shinozaki K (2004) Cloning and functional analysis of a novel DREB1/CBF transcription factor involved in cold-responsive gene expression in Zea mays. L Plant Cell Physiol 45: 1042–1052

    Article  PubMed  CAS  Google Scholar 

  • Qin F, Kakimoto M, Sakuma Y, Maruyama K, Osakabe Y, Tran LS, Shinozaki K, Yamaguchi-Shinozaki K (2007) Regulation and functional analysis of ZmDREB2A. in response to drought and heat stresses in Zea mays L Plant J 50: 54–69

    Article  PubMed  CAS  Google Scholar 

  • Sakuma Y, Liu Q, Dubouzet JG, Abe H, Shinozaki K, Yamaguchi-Shinozaki K (2002) DNA-binding specificity of the ERF/AP2 domain of Arabidopsis. DREBs, transcription factors involved in dehydration- and cold inducible gene expression Biochem Biophys Res Commun 290: 998–1009

    Article  PubMed  CAS  Google Scholar 

  • Sakuma Y, Maruyama K, Osakabe K, Qin F, Seki M, Shinozaki K, Yamaguchi-Shinozak K (2006a) Functional analysis of an Arabidopsis transcription factor, DREB2A, involved in drought-responsive gene expression. Plant Cell 18: 1292–1309

    Article  CAS  Google Scholar 

  • Sakuma Y, Maruyama K, Qin F, Osakabe Y, Shinozaki K, Yamaguchi-Shinozaki K (2006b). Dual function of an Arabidopsis. transcription factor DREB2A in water-stress- and heat-stress-responsive gene expression Proc Natl Acad Sc U S A 103: 18822–18827

    Article  CAS  Google Scholar 

  • Seki M, Narusaka M, Abe H, Kasuga M, Yamaguchi-Shinozaki K, Carninci P, Hayashizaki Y, Shinozaki K (2001) Monitoring the expression pattern of 1300 Arabidopsis genes under drought and cold stresses by using a full-length cDNA microarray. Plant Cell 13: 61–72

    Article  PubMed  CAS  Google Scholar 

  • Seki M, Narusaka M, Ishida J, Nanjo T, Fujita M, Oono Y, Kamiya A, Nakajima M, Enju A, Sakurai T, Satou M, Akiyama K, Taji T, Yamaguchi-Shinozaki K, Carninci P, Kawai J, Hayashizaki Y, Shinozaki K (2002) Monitoring the expression profiles of 7000 Arabidopsis. genes under drought, cold and high-salinity stresses using a full-length cDNA microarray Plant J 31: 279–292

    Article  PubMed  CAS  Google Scholar 

  • Seki M, Kamei A, Yamaguchi-Shinozaki K, Shinozaki K (2003) Molecular responses to drought, salinity and frost: common and different paths for plant protection. Curr Opin Biotechnol 14: 194–199

    Article  PubMed  CAS  Google Scholar 

  • Shinozaki K, Yamaguchi-Shinozaki K (2000) Molecular responses to dehydration and low temperature: differences and cross-talk between two stress signaling pathways. Curr Opin Plant Biol 3: 217–223

    PubMed  CAS  Google Scholar 

  • Shinozaki K, Yamaguchi-Shinozaki K, Seki M (2003) Regulatory network of gene expression in the drought and cold stress responses. Curr Opin Plant Biol 6: 410–417

    Article  PubMed  CAS  Google Scholar 

  • Shinwari ZK, Nakashima K, Miura S, Kasuga M, Seki M, Yamaguchi ShinozakiK, Shinozaki K (1998) An Arabidopsis gene family encoding DRE/CRT binding s involved in low-temperature-responsive gene expression. Biochem Biophys Res Commun 250: 161–170

    Article  PubMed  CAS  Google Scholar 

  • Stockinger EJ, Gilmour SJ, Thomashow MF (1997) Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit. Proc Natl Acad Sci U S A 94: 1035–1040

    Article  PubMed  CAS  Google Scholar 

  • Thomashow MF (1999) Plant cold acclimation: freezing tolerance genes and regulatory mechanisms Annu Rev Plant Physiol Plant Mol Biol 50: 571–599

    Article  PubMed  CAS  Google Scholar 

  • Vogel JT, Zarka DG, Van Buskirk HA, Fowler SG, Thomashow MF (2005) Roles of the CBF2 and ZAT12 transcription factors in configuring the low temperature transcriptome of Arabidopsis. Plant J 41: 195–211

    Article  PubMed  CAS  Google Scholar 

  • Yamaguchi-Shinozaki K, Shinozaki K (1994) A novel cis. -acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress Plant Cell 6: 251–264

    Article  PubMed  CAS  Google Scholar 

  • Yamaguchi-Shinozaki K, Shinozaki K (2006) Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stressed. Annu Rev Plant Biol 57: 781–803

    Article  PubMed  CAS  Google Scholar 

  • Zhang JZ, Creelman RA, Zhu JK (2004) From laboratory to field. Using information from Arabidopsis to engineer salt, cold, and drought tolerance in crops. Plant Physiol 135: 615–621

    CAS  Google Scholar 

  • Zhu J (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53: 247–273

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Plant Molecular Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, YayoiBunkyu-ku, Tokyo, 113-8657, Japan

    Kazuko Yamaguchi-Shinozaki

  2. Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, Ibaraki, 305-8686, Japan

    Kazuko Yamaguchi-Shinozaki

  3. RIKEN Plant Science Center, Tsurumi, Yokohama, Kanagawa, 230-0045, Japan

    Kazuo Shinozaki

  4. CREST, Japan Science and Technology Corporation (JST), Kawaguchi, Saitama, 332-0012, Japan

    Kazuko Yamaguchi-Shinozaki & Kazuo Shinozaki

Authors
  1. Kazuko Yamaguchi-Shinozaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kazuo Shinozaki
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Kazuko Yamaguchi-Shinozaki or Kazuo Shinozaki .

Rights and permissions

Reprints and permissions

Copyright information

© 2009 Springer Science + Business Media, LLC

About this paper

Cite this paper

Yamaguchi-Shinozaki, K., Shinozaki, K. (2009). DREB Regulons in Abiotic-Stress-Responsive Gene Expression in Plants. In: Molecular Breeding of Forage and Turf. Springer, New York, NY. https://doi.org/10.1007/978-0-387-79144-9_2

Download citation

Publish with us

Policies and ethics

Search

Navigation