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Handbook of Single-Molecule Biophysics pp 243–263Cite as

Precise Measurements of Diffusion in Solution by Fluorescence Correlations Spectroscopy

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Abstract

The chapter presents a general introduction to the method of fluorescence correlation spectroscopy (FCS) and its recently developed modification, dual-focus FCS. It explains the general optical setup of an FCS system, data acquisition, and data analysis. It discusses numerous potential optical and photophysical artifacts of FCS measurements and explains how these problems are circumvented by dual-focus FCS.

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Author information

Authors and Affiliations

  1. Drittes Physikalisches Institut, Georg-August-Universität Göttingen, Göttingen, Germany

    Jörg Enderlein

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  1. Jörg Enderlein
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Editors and Affiliations

  1. Institut für Biophysik, Universität Linz, Altenberger Straße 69, Linz, 4040, Austria

    Peter Hinterdorfer

  2. Harvard Medical School, Harvard University, Longwood Ave. 250, Boston, 02115, U.S.A.

    Antoine Oijen

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Enderlein, J. (2009). Precise Measurements of Diffusion in Solution by Fluorescence Correlations Spectroscopy. In: Hinterdorfer, P., Oijen, A. (eds) Handbook of Single-Molecule Biophysics. Springer, New York, NY. https://doi.org/10.1007/978-0-387-76497-9_9

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