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NASCA Microscopy: Super-Resolution Mapping of Chemical Reaction Centers

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Far-Field Optical Nanoscopy

Part of the book series: Springer Series on Fluorescence ((SS FLUOR,volume 14))

Abstract

Recently fluorescence microscopy has been introduced in the field of catalysis to study their dynamic molecular processes under in situ conditions with high spatial and temporal resolution. Because of the unique sensitivity down to the single molecule level, fluorescence microscopy allows to observe and localize chemical transformations with a subdiffraction-limited resolution. This chapter describes the use of fluorogenic probe molecules to visualize single chemical conversions using fluorescence microscopy. Special attention is paid to how visualization of single chemical conversions can yield super-resolution images beyond the diffraction limit.

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Abbreviations

BODIPY:

4,4-Difluoro-4-bora-3a,4a-diaza-s-indacene

GSDIM:

Ground-state depletion and single molecule return

LDH:

Layered double hydroxide

MRI:

Magnetic resonance imaging

NASCA:

Nanometer accuracy by stochastic chemical reactions

PALM:

Photo-activated localization microscopy

PET:

Photo-induced electron transfer

PSF:

Point-spread-function

STED:

Stimulated emission depletion microscopy

STORM:

Stochastic optical reconstruction microscopy

TIRF:

Total internal reflection fluorescence

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Author information

Authors and Affiliations

  1. Center for Surface Chemistry and Catalysis, Katholieke Universiteit Leuven, 3001, Heverlee, Belgium

    Gert De Cremer, Bert F. Sels, Dirk E. De Vos & Maarten B. J. Roeffaers

  2. Laboratory for Photochemistry and Spectroscopy, Katholieke Universiteit Leuven, 3001, Heverlee, Belgium

    Johan Hofkens & Maarten B. J. Roeffaers

Authors
  1. Gert De Cremer
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  2. Bert F. Sels
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  3. Dirk E. De Vos
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  4. Johan Hofkens
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  5. Maarten B. J. Roeffaers
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Corresponding author

Correspondence to Maarten B. J. Roeffaers .

Editor information

Editors and Affiliations

  1. NanoBioSciences Institute of Physical and Theoretical Chemistry, TU Braunschweig, Braunschweig, Germany

    Philip Tinnefeld

  2. MRC Human Immunology Unit and Wolfson Imaging Centre Oxford, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom

    Christian Eggeling

  3. Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany

    Stefan W. Hell

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© 2011 Springer-Verlag Berlin Heidelberg

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Cremer, G.D., Sels, B.F., Vos, D.E.D., Hofkens, J., Roeffaers, M.B.J. (2011). NASCA Microscopy: Super-Resolution Mapping of Chemical Reaction Centers. In: Tinnefeld, P., Eggeling, C., Hell, S. (eds) Far-Field Optical Nanoscopy. Springer Series on Fluorescence, vol 14. Springer, Berlin, Heidelberg. https://doi.org/10.1007/4243_2011_33

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