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EPR Spectroscopy pp 159–198Cite as

Structure and Dynamics of Nucleic Acids

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Part of the book series: Topics in Current Chemistry ((TOPCURRCHEM,volume 321))

Abstract

In this chapter we describe the application of CW and pulsed EPR methods for the investigation of structural and dynamical properties of RNA and DNA molecules and their interaction with small molecules and proteins. Special emphasis will be given to recent applications of dipolar spectroscopy on nucleic acids.

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Acknowledgments

Our own EPR work relied on the synthesis of spin-labeled RNA and DNA molecules performed by Nelly Piton and Olga Romainczyk from the group of Joachim W. Engels (Institute of Organic Chemistry and Chemical Biology, Goethe University Frankfurt) and the work by Pavol Cekan from the group of Snorri Th. Sigurdsson (University of Iceland) on the rigid spin labels for DNA. Vasyl Denysenkov is thanked for high-field G-band PELDOR experiments on DNA samples. Olav Schiemann (University of St. Andrews) is thanked for his major impact in the initial phase of this work as Habilitand in Frankfurt. Funding from the German Research Society (DFG) within the Collaborative Research Center 579 RNA-Ligand Interaction is gratefully acknowledged as well as support from the Center of Biomolecular Magnetic Resonance (BMRZ) and the Center of Excellence Frankfurt Macromolecular Complexes (DFG).

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  1. Institute of Physical and Theoretical Chemistry Center of Biomolecular Magnetic Resonance, Goethe University Frankfurt, Frankfurt am Main, Germany

    Ivan Krstić, Burkhard Endeward, Dominik Margraf, Andriy Marko & Thomas F. Prisner

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  1. Ivan Krstić
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  2. Burkhard Endeward
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  3. Dominik Margraf
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  4. Andriy Marko
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  5. Thomas F. Prisner
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Correspondence to Thomas F. Prisner .

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  1. , Fachbereich Chemie, University of Konstanz, Konstanz, 78457, Germany

    Malte Drescher

  2. , Physical Chemistry, ETH Zurich, Zurich, 8093, Switzerland

    Gunnar Jeschke

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Krstić, I., Endeward, B., Margraf, D., Marko, A., Prisner, T.F. (2011). Structure and Dynamics of Nucleic Acids. In: Drescher, M., Jeschke, G. (eds) EPR Spectroscopy. Topics in Current Chemistry, vol 321. Springer, Berlin, Heidelberg. https://doi.org/10.1007/128_2011_300

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