The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells

  • Jelena G. Najdanović
  • Stevo J. Najman
  • Sanja Stojanović
  • Jelena M. Živković
  • Nenad Ignjatović
  • Dragan Uskoković
  • Miroslav Trajanović
Conference paper

DOI: 10.2991/978-94-6239-213-7_27

Cite this paper as:
Najdanović J.G. et al. (2017) The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells. In: Lee B., Gadow R., Mitic V. (eds) Proceedings of the IV Advanced Ceramics and Applications Conference. Atlantis Press, Paris

Abstract

Nanomaterials are nowadays widely used in various fields of biomedicine. Before the application of biomaterials they have to be tested and fulfill some criteria. The first tests that should be performed after synthesis of some new nanomaterial with potential application in biomedical fields are biocompatibility tests. The aim of this study was to examine the influence of nanomaterial calcium phosphate/poly-(dl-lactide-co-glycolide) (CP/PLGA) on proliferation and adherence of HeLa cells in culture. For both proliferation and adherence examination, cells were treated with suspension of nanomaterial CP/PLGA at concentrations 5, 50 and 500 µg/mL, made in cell culture medium. There were four different types of treatment: (1) cells incubated with CP/PLGA for 3 days; (2) cells incubated with CP/PLGA for 6 days; (3) cells incubated first with CP/PLGA for 3 days and then for the next 3 days with medium and (4) cells incubated first with medium for 3 days and then for the next 3 days with CP/PLGA. Three days after incubation of HeLa cells with different concentrations of CP/PLGA nanoparticles’ suspension, the concentration of 5 µg/mL had mild inhibitory effect on proliferation. Increasing CP/PLGA concentration, there was stimulatory effect on cells’ proliferation. With prolonged incubation period, this dose dependence is lost. The highest adherence of HeLa cells was observed when cells were incubated with the highest examined concentration of CP/PLGA suspension, in both 3-day and 6-day incubation period. Based on the results obtained in our study, we can conclude that the effect of the suspension of nanomaterial CP/PLGA on proliferation and adherence of HeLa cells depends on the duration of incubation with the cells as well as the material concentration and type of the treatment.

Keywords

Nanomaterial CP/PLGA Hela cells Proliferation Adherence 

Copyright information

© Atlantis Press and the author(s) 2017

Authors and Affiliations

  • Jelena G. Najdanović
    • 1
  • Stevo J. Najman
    • 1
  • Sanja Stojanović
    • 1
  • Jelena M. Živković
    • 1
  • Nenad Ignjatović
    • 2
  • Dragan Uskoković
    • 2
  • Miroslav Trajanović
    • 3
  1. 1.Faculty of MedicineUniversity of NišNišSerbia
  2. 2.Institute of Technical Sciences of the Serbian Academy of Sciences and ArtsBelgradeSerbia
  3. 3.Faculty of Mechanical EngineeringUniversity of NišNišSerbia