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Molecular Diagnostics pp 163–172Cite as

Flow Cytometry

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Abstract

1. General Principles Of Flow Cytometry Flow cytometry is a technique in which single cells in a fluid suspension are analyzed with respect to their intrinsic lightscattering properties and are simultaneously evaluated for one or more extrinsic properties (i.e., the presence of specific molecules) using fluorescent probes. The fluorescent probe might bind directly to the targeted molecule (e.g., propidium iodide in DNA content analysis) or a fluorescent dye might be coupled to an antibody probe, to enable detection of a specific protein. By using several different fluorochromes with substantially nonoverlapping emission spectra, the laboratorian can simultaneously evaluate the expression of multiple extrinsic cellular properties. Moreover, because the flow rate of cells within the flow cytometer is rapid, thousands of cells can be analyzed in seconds. Despite the rapidity with which data can be acquired, however, because each cell is analyzed individually, multiple intrinsic and extrinsic parameters are retained for each cell; that is, flow cytometric analysis is multiparametric. In this section, we review some general principles of flow cytometry (1–6).

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Author information

Authors and Affiliations

  1. Special Hematology Laboratory, Acting Director, Molecular Pathology Laboratory, Department of Pathology and Laboratory Medicine, Hartford Hospital, Hartford, CT

    Joseph A. DiGiuseppe MD, PhD (Director)

Authors
  1. Joseph A. DiGiuseppe MD, PhD
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Editor information

Editors and Affiliations

  1. Department of Pathology and Laboratory Medicine, University of North Carolina School of Medicine, Chapel Hill, NC, USA

    William B. Coleman

  2. Department of Pathology, Dartmouth-Hitchcock Medical Center, Lebanon, NH, USA

    Gregory J. Tsongalis

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© 2006 Humana Press, a part of Springer Science+Business Media, LLC

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DiGiuseppe, J.A. (2006). Flow Cytometry. In: Coleman, W.B., Tsongalis, G.J. (eds) Molecular Diagnostics. Humana Press. https://doi.org/10.1385/1-59259-928-1:163

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  • DOI: https://doi.org/10.1385/1-59259-928-1:163

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-356-5

  • Online ISBN: 978-1-59259-928-8

  • eBook Packages: MedicineMedicine (R0)

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