In vitro and in vivo Evaluation of a Plasmid Encoding Canine Interleukin 12

  • U. Lampreht
  • U. Kamensek
  • M. Stimac
  • M. Bosnjak
  • S. Kranjc
  • G. Sersa
  • M. Cemazar
Conference paper

DOI: 10.1007/978-981-287-817-5_96

Volume 53 of the book series IFMBE Proceedings (IFMBE)
Cite this paper as:
Lampreht U. et al. (2016) In vitro and in vivo Evaluation of a Plasmid Encoding Canine Interleukin 12. In: Jarm T., Kramar P. (eds) 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies. IFMBE Proceedings, vol 53. Springer, Singapore

Abstract

Clinical studies using gene electrotransfer of plasmid encoding human interleukin 12 (IL-12) have already shown good results when treating tumors in canine patients. Nevertheless, the use of a plasmid encoding canine IL-12 is essential for further clinical trials. Therefore, the aim of this study was to evaluate the effect of the plasmid encoding canine IL-12 in in vitro and in vivo studies and compare its activity to the plasmid encoding the human IL-12. Our results showed that after in vitro gene electrotransfer plasmid encoding canine IL-12 had similar or better IL-12 expression capacity than plasmid encoding human IL-12. Further on, the in vivo experiment in nude mice revealed that the therapeutic effect of plasmid encoding canine IL-12 after gene electrotransfer was comparable to the therapeutic effect of plasmid encoding human IL-12 after gene electrotransfer. Also, the effect on tumor growth delay was equivalent after first and second therapy, which shows that the gene electrotherapy of plasmid encoding canine IL-12 is repeatable. Therefore, our study suggests that the plasmid encoding canine IL-12 is suitable for further clinical studies in dogs.

Keywords

gene electrotransfer interleukin 12 veterinary oncology canine malignant melanoma cells 

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Copyright information

© Springer Science+Business Media Singapore 2016

Authors and Affiliations

  • U. Lampreht
    • 1
  • U. Kamensek
    • 1
  • M. Stimac
    • 1
  • M. Bosnjak
    • 1
  • S. Kranjc
    • 1
  • G. Sersa
    • 1
  • M. Cemazar
    • 1
    • 2
  1. 1.Department of experimental oncologyInstitute of Oncology LjubljanaLjubljanaSlovenia
  2. 2.Faculty of Health SciencesUniversity of PrimorskaIzolaSlovenia