Abstract
A recently developed prototype of a microfluidic cell sorting system fabricated on silicon wafers using semiconductor technology is presented. Preliminary results show that we can successfully sort fluorescent particles from non-fluorescent particles. When the performance of the structure has been optimised we will initiate sorting of fluorescently labelled foetal cells. Sorting foetal cells from mother’s blood offers a safer alternative to currently used methods of prenatal genetic diagnostic screening that carry a substantial risk of harming the foetus and may even lead to pregnancy loss.
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References
Tabor A, Philip J, Madsen M, Bang J, Obel EB, Norgaard-Pedersen B, Randomised controlled trial of genetic amniocentesis in 4606 low-risk women,.Lancet 1986 Jun 7;1 (8493):1287–1293
Zheng YL, Carter NP, Price CM, Colman SM, Milton PJ, Hackett GA, Greaves MF, Ferguson-Smith MA, Prenatal diagnosis from maternal blood: simultaneous immunophenotyping and FISH of fetal nucleated erythrocytes isolated by negative magnetic cell sorting, J Med Genet, 12 (1993) 1051–1056
J.K. Larsen, Principles, capacity and limitation of immunofluorescent flow sorting of rare cell species, In Hematopoietic stem cells:The Mulhouse Manual, E. Wunder, H. Solvalat, P. Henon, S. Sweke (eds) AlphaMed Press, Dayton, Ohio (1994) 133–140.
G. Blankenstein and U.D. Larsen, Modular concept of a laboratory on a chip for chemical and biochemical analysis, Biosensors & Bioelectronics, 13 (1998) 427–438.
Y.-L. Zheng, D.K. Zhen, M.A. DeMaria, S.M. Berry, R.J. Wapner, M.I. Evans, D. Copeland, J.M. Williams, D.W. Bianchi, Search for the optimal fetal cell antibody: results of immunophenotyping studies using flow cytometry, Hum. Gent, 100 (1997) 35–42.
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© 1998 Springer Science+Business Media Dordrecht
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Wolff, A., Larsen, U.D., Blankenstein, G., Philip, J., Telleman, P. (1998). Rare Event Cell Surging in a Microfluidic System for Application in Prenatal Diagnosis. In: Harrison, D.J., van den Berg, A. (eds) Micro Total Analysis Systems ’98. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5286-0_18
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DOI: https://doi.org/10.1007/978-94-011-5286-0_18
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6225-1
Online ISBN: 978-94-011-5286-0
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