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Mononuclear Phagocytes pp 9–18Cite as

The mononuclear phagocyte system of the mouse defined by immunohistochemical localisation of antigen F4/80

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Abstract

The mononuclear phagocyte system (MPS) is defined as a population of cells comprising bone marrow progenitor cells, blood monocytes and tissue macrophages (1). Blood monocytes and mature macrophages display considerably diversity of phenotype and function (2–5), some of which may arise at the level of committed bone marrow progenitor cells (3, 6, 7). There is no evidence, to our knowledge, that macrophage heterogeneity arises prior to the formation of the CFUG, M, the earliest committed progenitor that can give rise to mixed colonies of granulocytes and macrophages (8, 9). A functional definition of the mononuclear phagocyte system as being those cells that are derived from CFUG, M but are not granulocytes is of little experimental value. For most purposes mononuclear phagocytes are defined by adherence, morphology, lysozyme secretion, Fc and C3 receptors and expression of non-specific esterase, lysosomal hydrolases (esp. acid phosphatase) and ectoenzymes (2, 4, 5). The deficiencies in these criteria have lent impetus to attempts to obtain monoclonal antibodies that recognize mononuclear phagocytes. Several antibodies against mouse macrophages have been produced (10–18) but most, like previous markers, are either expressed on other cell types or are absent from a subpopulation of macrophages. In this paper we review our studies on the mouse antigen F4/80 (18) and its use as a marker for mononuclear phagocytes.

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Authors
  1. D. A. Hume
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  2. S. Gordon
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Editors and Affiliations

  1. Department of Infectious Diseases, University Hospital, Rijnsburgerweg 10, 2333 AA, Leiden, The Netherlands

    Ralph van Furth

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© 1985 Martinus Nijhoff Publishers, Dordrecht

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Hume, D.A., Gordon, S. (1985). The mononuclear phagocyte system of the mouse defined by immunohistochemical localisation of antigen F4/80. In: van Furth, R. (eds) Mononuclear Phagocytes. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-5020-7_2

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  • DOI: https://doi.org/10.1007/978-94-009-5020-7_2

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-8723-0

  • Online ISBN: 978-94-009-5020-7

  • eBook Packages: Springer Book Archive

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