Abstract
Chloroplast DNA (cpDNA) is a small highly conserved cytoplasmic molecule which has been successfully used in inter- and intraspecific phylogenetic studies (1). Moreover, in most plant species, cpDNA has a monoparental inheritance and its geographical variation is usually very different from that obtained using nuclear markers. Variation of cpDNA has mostly been studied in herbaceous species and very few papers have dealt with ligneous species. These are often high in secondary compounds and are considered therefore as a difficult material for cpDNA extraction. This method was devised to analyse chloroplast DNA in species high in phenolics and polysaccharides and is particularly suitable for sclerophyllous broad-leaved trees. It includes two major steps.
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Lumaret, R., Michaud, H., Ripoll, JP., Toumi, L. (1998). Chloroplast DNA Extraction Procedure for Species High in Phenolics and Polysaccharides. In: Karp, A., Isaac, P.G., Ingram, D.S. (eds) Molecular Tools for Screening Biodiversity. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0019-6_3
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DOI: https://doi.org/10.1007/978-94-009-0019-6_3
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6496-5
Online ISBN: 978-94-009-0019-6
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