Isolation of Nucleic Acids Using Anion-Exchange Chromatography: QIAGEN-tip Based Methods

Abstract

Nucleic acids are highly charged, linear polyanions and can therefore be separated from other components by anion-exchange chromatography The QIAGEN Resin is a macro-porous anion-exchanger with a particle size of approximately 100 µm, and a hydrophilic surface coating that allows dense coupling of diethylaminoethyl groups. The large pore size, together with the high density of anion-exchange groups, provides a broad separation range that allows selective separation of nucleic acids from proteins, polysaccharides and metabolites. It also allows the separation of different classes of nucleic acids from each other by successive elution steps using simple salt buffers. The separation range of QIAGEN Resin (from 0.1 M to 1.6 M salt; see Fig. 4.1.2) is very broad compared with conventional anion-exchange resins (usually from 0.1 to 0.6 M salt).