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Hexanoyl-Lysine as a Deterioration Marker for Rice During Storage

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Lipid Hydroperoxide-Derived Modification of Biomolecules

Part of the book series: Subcellular Biochemistry ((SCBI,volume 77))

Abstract

Nε-(hexanoyl)lysine (HEL) is known to be an oxidative lipid-decomposition product, and a powerful marker indicating oxidative stress in animal tissue. We investigated whether HEL could be useful as a marker in rice seeds damaged by oxidative stress during storage, as well as animals. We could show an accumulation of HEL in rice stored at high temperature (40 °C). This result significantly corresponded with an accumulation of TBARS. Rice germination deteriorated with non-enzymatic lipid peroxidation during storage at high temperature for a few months. These results suggested that a deterioration of rice germination ability resulted from oxidative damage caused by lipid peroxidation during storage. Moreover, HEL could become a useful marker for oxidative stress induced by lipid peroxidation. In addition, the activities of antioxidant enzymes, catalase and superoxide dismutase, significantly decreased in the rice seeds during storage at 40 °C. The relationship between accumulation of HEL and increases in antioxidant enzymes activities must be further studied. But, these results suggest that HEL might be a useful marker of oxidative stress in rice as well as in animals.

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Abbreviations

HEL:

Nε-(hexanoyl)lysine

DT:

o, o-dityrosine

NT:

Nitrotyrosine

TBARS:

Thiobarbituric acid-reactive substance

ELISA:

Enzyme-linked immunosorbent assay

AOS:

Activated oxygen species

LOX:

Lipoxygenase

CAT:

Catalase

Mn-SOD:

Manganese – superoxide dismutase

Cu, Zn-SOC:

Copper, Zinc – superoxide dismutase

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Acknowledgment

I am deeply grateful to Professor Toshihiko Osawa of Aichi-gakuin University and Professor Yoji Kato of University of Hyogo for their guidance and support.

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Authors and Affiliations

  1. Department of Applied Biological Chemistry, Meijo University, 1-501 Shiogamaguchi, Nagoya, 468-8502, Japan

    Ken-ichiro Minato

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  1. Ken-ichiro Minato
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Correspondence to Ken-ichiro Minato .

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Editors and Affiliations

  1. School of Human Science & Environment, University of Hyogo, Himeji, Hyogo, Japan

    Yoji Kato

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Minato, Ki. (2014). Hexanoyl-Lysine as a Deterioration Marker for Rice During Storage. In: Kato, Y. (eds) Lipid Hydroperoxide-Derived Modification of Biomolecules. Subcellular Biochemistry, vol 77. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7920-4_6

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