Abstract
Gene engineering, also called genetic engineering or recombinant DNA technique, has been a newly emerging technology since 1970s. Its principle is to artificially isolate biological genetic materials (usually DNA) for their being cleaved, incorporated, restructured, transferred and expressed in vitro. Generally, it involves four steps. The first step is the cloning of the target gene to obtain the needed DNA segments. The second step is to obtain the recombinant DNA by connecting the target gene with DNA vector. The third step is to introduce the recombinant DNA into the bacteria or mammalian cells for its proliferation. And the last step is to screen the receptor cells that express the target genes for expression of the corresponding proteins and other products. The recombinant DNA technique is one of the most commonly used technologies in molecular biology, which has been widely applied in scientific research and the clinical detection of HIV.
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References
Church JD et al. Comparison of laboratory methods for analysis of non-nucleoside reverse transcriptase inhibitor resistance in Ugandan infants. AIDS Res Hum Retroviruses. 2009;25(7):657–63.
Kellam P, Larder BA. Recominant virus assay: a rapid, phenotypic assay for assessment of drug susceptibility of human immunodeficiency virus type 1 isolates. Antimicrob Agents Chemother. 1994;38(1):23–30.
Drmanac S et al. Accurate sequencing by hybridization for DNA diagnostics and individual genomics. Nat Biotechnol. 1998;16(1):54–8.
Petropoulos CJ et al. A novel phenotypic drug susceptibility assay for human immunodeficiency virus type 1. Antimicrob Agents Chemother. 2000;44(4):920–8.
Descamps D et al. Line probe assay for detection of human immunodeficiency virus type 1 mutations conferring resistance to nucleoside inhibitors of reverse transcriptase: comparison with sequence analysis. J Clin Microbiol. 1998;36(7):2143–5.
Vahey M et al. Performance of the Affymetrix GeneChip HIV PRT 440 platform for antiretrovital drug resistance genotyping of human immunodeficiency virus type 1 clades and viral isolates with length polymorphisms. J Clin Microbiol. 1999;37(8):2533–7.
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© 2014 Springer Science+Business Media Dordrecht and People’s Medical Publishing House
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Li, H. (2014). Molecular Biology of HIV/AIDS and Its Clinical Management. In: Li, H. (eds) Radiology of HIV/AIDS. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7823-8_1
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DOI: https://doi.org/10.1007/978-94-007-7823-8_1
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