Molecular Cloning, Sequence Analysis of Thioesterases from Wintersweet (Chimonanthus Praecox)

  • Li-Hong Zhang
  • Qiong Wu
  • Xian-Feng Zou
  • Li-Na Chen
  • Shu-Yan Yu
  • Chang-Cheng Gao
  • Xing Chen
Conference paper

DOI: 10.1007/978-94-007-7618-0_327

Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 269)
Cite this paper as:
Zhang LH. et al. (2014) Molecular Cloning, Sequence Analysis of Thioesterases from Wintersweet (Chimonanthus Praecox). In: Li S., Jin Q., Jiang X., Park J. (eds) Frontier and Future Development of Information Technology in Medicine and Education. Lecture Notes in Electrical Engineering, vol 269. Springer, Dordrecht

Abstract

A coding region of wintersweet cDNA was cloned via screening a wintersweet cDNA library. The CpFATB cDNA is 1110 bp in length with an open reading frame (ORF) of 1107 bp encoding a protein of 369 amino acids. Molecular weight and isoelectric point of the protein is 41.72 kD and 7.72, respectively. Bioinformatics analysis shows that a signal peptide with 49 amino acid residues and Protein Localization Sites exists in the chloroplast stroma. This classifies the protein as stable.

Keywords

Thioesterases Bioinformatics Wintersweet 

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Li-Hong Zhang
    • 1
  • Qiong Wu
    • 1
  • Xian-Feng Zou
    • 1
  • Li-Na Chen
    • 1
  • Shu-Yan Yu
    • 1
  • Chang-Cheng Gao
    • 1
  • Xing Chen
    • 1
  1. 1.Key Laboratory of Agricultural Products ProcessingChangchun UniversityChangchunPeople’s Republic of China

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