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The gp82 Surface Molecule of Trypanosoma cruzi Metacyclic Forms

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Book cover Proteins and Proteomics of Leishmania and Trypanosoma

Part of the book series: Subcellular Biochemistry ((SCBI,volume 74))

Abstract

Gp82 is a surface glycoprotein expressed in Trypanosoma cruzi metacyclic trypomastigotes, the parasite forms from the insect vector that initiate infection in the mammalian host. Studies with metacyclic forms generated in vitro, as counterparts of insect-borne parasites, have shown that gp82 plays an essential role in host cell invasion and in the establishment of infection by the oral route. Among the gp82 properties relevant for infection are the gastric mucin-binding capacity and the ability to induce the target cell signaling cascades that result in actin cytoskeleton disruption and lysosome exocytosis, events that facilitate parasite internalization. The gp82 sequences from genetically divergent T. cruzi strains are highly conserved, displaying >90 % identity. Both the host cell-binding sites, as well as the gastric mucin-binding sequence of gp82, are localized in the C-terminal domain of the molecule. In the gp82 structure model, the main cell-binding site consists of an α-helix, which connects the N-terminal β-propeller domain to the C-terminal β-sandwich domain, where the second cell binding site is nested. The two cell binding sites are fully exposed on gp82 surface. Downstream and close to the α-helix is the gp82 gastric mucin-binding site, which is partially exposed. All available data support the notion that gp82 is structurally suited for metacyclic trypomastigote invasion of host cells and for initiating infection by the oral route.

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Abbreviations

ACD:

Acute Chagas’ disease

DAG:

Diacylglycerol

EIEC:

Enteroinvasive Escherichia coli

GST:

Glutathione S transferase

IP3:

Inositol 1,4,5-triphosphate

MT:

Metacyclic trypomastigotes

mTOR:

Mammalian target of rapamycin

PI3K:

Phosphatidylinositol 3-kinase

PTK:

Protein tyrosine kinase

TCT:

Tissue culture trypomastigotes

PKC:

Protein kinase C

PLC:

Phospholipase C

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Acknowledgements

 Work supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and Conselho Nacional de Desenvolvimento Cientfico e Tecnológico (CNPq).

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Authors and Affiliations

  1. Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, R. Pedro de Toledo, 669 – 6º andar, 04039-032, São Paulo, SP, Brazil

    Cristian Cortez, Fernando Y. Maeda & Nobuko Yoshida

  2. Laboratório Nacional de Biociências, Centro Nacional de Pesquisa em Energia e Materiais, Campinas, Brazil

    Tiago J. P. Sobreira

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  1. Cristian Cortez
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  2. Tiago J. P. Sobreira
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  3. Fernando Y. Maeda
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  4. Nobuko Yoshida
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Correspondence to Nobuko Yoshida .

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Editors and Affiliations

  1. Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil

    André L.S. Santos

  2. Departamento de Microbiologia Geral, Ins. de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil

    Marta H. Branquinha

  3. Fundação Oswaldo Cruz, Rio de Janeiro, Rio de Janeiro, Brazil

    Claudia M. d’Avila-Levy

  4. Fundação Oswaldo Cruz, Rio de Janeiro, Rio de Janeiro, Brazil

    Lucimar F. Kneipp

  5. Departamento de Biologia Celular e Molecular, Instituto de Biologia Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil

    Cátia L. Sodré

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Cortez, C., Sobreira, T.J.P., Maeda, F.Y., Yoshida, N. (2014). The gp82 Surface Molecule of Trypanosoma cruzi Metacyclic Forms. In: Santos, A., Branquinha, M., d’Avila-Levy, C., Kneipp, L., Sodré, C. (eds) Proteins and Proteomics of Leishmania and Trypanosoma. Subcellular Biochemistry, vol 74. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7305-9_6

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