Abstract
A virus particle must work as a safe box for protecting its genome, but at the same time it has to undergo dramatic conformational changes in order to preserve itself by propagating in a cell infection. Thus, viruses are miniaturized wonders whose structural complexity requires them to be investigated by a combination of different techniques that can tackle both static and dynamic processes. In this chapter we will illustrate how major structural techniques such as X-ray crystallography and electron microscopy have been and can be combined with other techniques to determine the structure of complex viruses. The power of these hybrid method approaches are revealed through the various examples provided.
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Notes
- 1.
Also especially recommended for further reading are references [7, 9, 13, 25, 27, 99, 112] listed above.
Abbreviations
- 2D:
-
Two dimensional
- 3D:
-
Three dimensional
- BMV:
-
Bromegrass mosaic virus
- EM:
-
Electron microscopy
- ET:
-
Electron tomography
- Fab:
-
Antigen-binding antibody fragment
- FMDV:
-
Foot-and-mouth disease virus
- HCV:
-
Hepatitis C virus
- HCV-LP:
-
HCV-like particle
- HIV:
-
Human immunodeficiency virus
- HRV-16:
-
Human rhinovirus 16
- HSV:
-
Herpes simplex virus
- MR:
-
Molecular replacement
- NCS:
-
Non-crystallographic symmetry
- NMR:
-
Nuclear magnetic resonance
- RVFV:
-
Rift Valley fever virus
- SANS:
-
Small-angle neutron scattering
- SAXS:
-
Small-angle X-ray scattering
- SBMV:
-
Southern bean mosaic virus
- SeMet:
-
Seleno-methionine
- SIV:
-
Simian immunodeficiency virus
- TBSV:
-
Tomato bushy stunt virus
- TEM:
-
Transmission electron microscopy
- TMV:
-
Tobacco mosaic virus
- TNV:
-
Tobacco necrosis virus
- VLP:
-
Virus-like particle
- WNV:
-
West Nile virus
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Further Reading
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Acknowledgements
DB acknowledges support from the CIC bioGUNE and HMO thanks the University of Helsinki for funding to EU ESFRI Instruct Centre for Virus Production and Purification. DIS is supported by the UK MRC. This work was enabled by the Spanish Ministerio de Ciencia y Innovacion (BFU2009-08123), Spanish Ministerio de Economia y Competitividad (BFU2012-33947) and the Basque Government (PI2010-20) grants to NGAA.
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Badia-Martinez, D., Oksanen, H.M., Stuart, D.I., Abrescia, N.G.A. (2013). Combined Approaches to Study Virus Structures. In: Mateu, M. (eds) Structure and Physics of Viruses. Subcellular Biochemistry, vol 68. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-6552-8_7
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