Abstract
Chromatographic methods for chlorophenols (CPs) determination that can be applied to clinical (blood, urine) and breast milk samples are reviewed. Special emphasis is given to sample storage conditions and to the application of preconcentration techniques. Solid phase extraction, solid phase microextraction, stir-bar sorptive extraction, liquid phase microextraction and steam distillation methods are considered in view of the potential use of CPs as biomarkers of exposure to different pesticides.
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Bartels P, Edeling E, Krämer B, Kruse H, Osius N, Vowinkel K, Wassermann O, Witten J, Zorn C (1999) Determination of chlorophenols in urine of children and suggestion of reference values. Fresenius J Anal Chem 365:458–464
Becker K, Schulz C, Kaus S, Seiwert M, Seifert B (2003) German environmental survey 1998 (GerES III): environmental pollutants in the urine of the German population. Int J Hyg Environ Health 206:15–24
Collins JJ, Bodner K, Haidar S, Wilken M, Burns CJ, Lamparski L, Budinsky RA, Martin GD, Carson ML (2008) Chlorinated dibenzo-p-dioxins, dibenzofurans and biphenyl profiles of workers with trichlorophenol and pentachlorophenol exposures. Chemosphere 73:S284–S289
Crespin MA, Gallego M, Valcarcel M (2002) Solid-phase extraction method for the determination of free and conjugated phenol compounds in human urine. J Chromatogr B 773:89–96
de Morais P, Stoichev T, Basto MCP, Vasconcelos MTSD (2012) Extraction and preconcentration techniques for chromatographic determination of chlorophenols in environmental and food samples. Talanta 89:1–11
Guidotti M, Ravaioli G, Vitali M (1999) Total p-chlorophenol determination in urine samples of subjects exposed to chlorobenzene using SPME and GC-MS. J High Resol Chromatogr 22:427–428
Hill RH, Ashley DL, Head SL, Needham LL, Prike JL (1995b) p-Dichlorobenzene exposure among 1000 adults in the United States. Arch Environ Health 50:277–280
Hill RH, Head SL, Baker S, Gregg M, Shealy DB, Bailey SL, Williams CC, Sampson EJ, Needham LL (1995a) Pesticide residues in the urine of adults living in the United States: reference range concentrations. Environ Res 71:99–108
Hsiao P-K, Lin Y-C, Shih T-S, Chiung Y-M (2009) Effects of occupational exposure to 1,4-dichlorophenzene on hematologic, kidney and liver functions. Int Arch Occup Environ Health 82:1077–1085
Ito R, Kawaguchi M, Honda H, Koganei Y, Okanouchi N, Sakui N, Saito K, Nakazawa H (2008) Hollow-fiber-supported liquid phase microextraction with in situ derivatization and gas chromatography mass spectrometry for determination of chlorophenols in human urine samples. J Chromatogr B 872:63–67
Kawaguchi M, Ishii Y, Sakui N, Okanouchi N, Ito R, Saito K, Nakazawa H (2005) Stir bar sorptive extraction with in situ derivatization and thermal desorption gas chromatography–mass spectrometry for determination of chlorophenols in water and body fluid samples. Anal Chim Acta 533:57–65
Kontsas H, Rosenberg C, Pfäffi P, Jäppinen P (1995) Gas chromatographic-mass spectrometric determination of chlorophenols in the urine of sawmill workers with past use of chlorophenol-containing antistain agents. Analyst 120:1745–1749
Kumagai S, Matsunaga I (1994) Concentrations of urinary metabolites in workers exposed to monochlorobenzene and variation in the concentration during a work shift. Occup Environ Med 51:120–124
Kumagai S, Matsunaga I (1997) Quantitative determination of urinary metabolites of o-dichlorobenzene using a gas chromatograph. Industr Health 35:399–403
Kurihara N, Nakajima M (1974) Studies on BHC isomers and related compounds: urinary metabolites produced from γ- and β-BHC in the mouse: chlorophenol conjugates. Pesticide Biochem Physiol 4:220–231
Lee M-R, Yeh Y-C, Hsiang W-S, Chen C-C (1998) Application of solid-phase microextraction and gas chromatography–mass spectrometry for the determination of chlorophenols in urine. J Chromatogr B 707:91–97
Marsden PJ, Amick EN, Shore FL, Williams LR, Bohman VR, Blincoe CR (1986) Characterization of bovine urine and adipose interlaboratory performance evaluation samples containing biologically incorporated chlorophenols. J Agric Food Chem 34:795–800
Navarro-Villoslada F, Pérez-Arribas LV, León-González ME, Polo-Diez LM (1999) Matrix effect modeling in multivariate determination of priority pollutant chlorophenols in urine samples. Anal Chim Acta 381:93–102
Norberg J, Emnéus J, Jönsson JA, Mathiasson L, Burestedt E, Knutsson M, Marko-Varga G (1997) On-line supported liquid membrane-liquid chromatography with a phenol oxidase-based biosensor as a selective detection unit for the determination of phenols in blood plasma. J Chromatogr B 701:39–46
Rick DL, Licht CF, McCarty LP (1982) Determination of phenol and pentachlorophenol in plasma and urine samples by gas liquid chromatography. J Anal Toxicol 6:297–300
Röhrig L, Meisch H-U (2000) Application of solid phase microextraction for the rapid analysis of chlorinated organics in breast milk. Fresenius J Anal Chem 366:106–111
Röhrig L, Püttmann M, Meisch H-U (1998) Determination of persistent organochlorine compounds in blood by solid phase microextraction and GC-ECD. Fresenius J Anal Chem 361:192–196
Veningerová M, Uhnák J, Prachar V, Kovacicová J (1996) Chlorinated phenols in human milk. Z Lebensm Unters Forsch 203:309–310
Wada M, Kinoshita S, Itayama Y, Kuroda N, Nakashima K (1999) Sensitive high-performance liquid chromatographic determination with fluorescence detection of phenol and chlorophenols with 4-(4,5-diphenyl-1H-imidazol-2-yl) benzoyl chloride as a labeling reagent. J Chromatogr B 721:179–186
Zhou Y, Jiang Q, Peng Q, Xuan D, Qu W (2007) Development of a solid phase microextraction-gas chromatography–mass spectrometry method for the determination of pentachlorophenol in human plasma using experimental design. Chemosphere 70:256–262
Acknowledgements
Funds from CIIMAR (PesT-C/MAR/LA0015/2011) and the Foundation for Science and Technology (Portugal) are acknowledged for the fellowships awarded to P. de Morais (SFRH/BD/66492/2009) and T. Stoichev (SFRH/BPD/32700/2006) co-financed by POPH/FSE.
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de Morais, P., Stoichev, T., Basto, M.C., Vasconcelos, M.T. (2013). Chromatographic Methods for Determination of Chlorophenols as Biomarkers of Exposure to Pesticides and Organochlorine Substances. In: Simeonov, L., Macaev, F., Simeonova, B. (eds) Environmental Security Assessment and Management of Obsolete Pesticides in Southeast Europe. NATO Science for Peace and Security Series C: Environmental Security. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-6461-3_18
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