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Tetraspanins pp 91–108Cite as

Dynamic Partitioning of Tetraspanins Within Plasma Membranes

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Part of the book series: Proteins and Cell Regulation ((PROR,volume 9))

Abstract

The study of the organization and dynamics of biological membranes has greatly benefited from the considerable technical advances achieved in the photonics field. Breaking the diffraction limit in optical microscopy to further increase spatial resolution has allowed to define the ultra-structure of the different proteolipid complexes within cellular membranes. Furthermore, the improvements in fluorescence sensitivity have prompted to the analysis of molecular dynamics up to single-molecule level with high temporal resolution. Thanks to all these advances, the concept of tetraspanin-enriched microdomains has been revisited in recent studies that shed new light on tetraspanin dynamics and interactions.

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Abbreviations

Chl:

Cholesterol

FCCS:

Fluorescence Cross-Correlation Spectroscopy

FCS:

Fluorescence Correlation Spectroscopy

FRAP:

Fluorescent Recovery After Photobleaching

FRET-FLIM:

Fluorescence Resonance Energy Transfer-Fluorescence Lifetime Imaging Microscopy

PALM:

Photo-Activated Localization Microscopy

SMT:

Single Molecule Tracking

STED:

Stimulated Emission Depletion

STORM:

Stochastic Optical Reconstruction Microscopy

TEM:

Tetraspanin-Enriched Microdomain

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Acknowledgements

We would like to thank Dr. Moreno Zamai for participating in data acquisition and analysis of Fig. 4.1. This work was supported by the Spanish Ministry of Science and Innovation (SAF2008-06235); and MEICA from Genoma España Foundation, INSINET from Comunidad Autónoma de Madrid [FONCICYT-C002-2009-1ALA/127249], and RECAVA [RD06/0014-0030] from Instituto de Salud Carlos III to F.S-M. This work was also supported by the Association Nationale pour la Recherche (ANR-06-BLAN-0378) to P.E.M. O.B. is a post-­doctoral fellow from Spanish Ministry of Science and Innovation ‘Juan de la Cierva Program’(JDC 08). Centro Nacional de Investigaciones Cardiovasculares is supported by the Ministry of Science and Innovation and the Pro CNIC Foundation.

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Authors and Affiliations

  1. Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain

    Olga Barreiro & Francisco Sanchez-Madrid

  2. Servicio de Inmunología, Hospital Universitario de la Princesa, Instituto de Investigación Sanitaria Princesa (IP), Madrid, Spain

    Olga Barreiro & Francisco Sanchez-Madrid

  3. Department of Cell and Developmental Biology, Weill Medical College, Cornell University, New York, USA

    Cédric Espenel

  4. Centre de Biochimie Structurale, CNRS UMR 5048, INSERM U1054, UM1, UM2, Montpellier, France

    Pierre-Emmanuel Milhiet

Authors
  1. Olga Barreiro
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  2. Francisco Sanchez-Madrid
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  3. Cédric Espenel
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  4. Pierre-Emmanuel Milhiet
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Correspondence to Olga Barreiro or Pierre-Emmanuel Milhiet .

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Editors and Affiliations

  1. , School of Cancer Sciences, University of Birmingham, Vincent Drive, Birmingham, B15 2TT, United Kingdom

    Fedor Berditchevski

  2. INSERM U1004, Av. Paul Vaillant Couturier 14, Villejuif, F-94807, France

    Eric Rubinstein

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Barreiro, O., Sanchez-Madrid, F., Espenel, C., Milhiet, PE. (2013). Dynamic Partitioning of Tetraspanins Within Plasma Membranes. In: Berditchevski, F., Rubinstein, E. (eds) Tetraspanins. Proteins and Cell Regulation, vol 9. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-6070-7_4

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