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Biotechnology of Neglected and Underutilized Crops pp 203–216Cite as

Biotechnology of Eruca Sativa Mill

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Abstract

Eruca sativa Mill is amenable to biotechnological procedures and these initial protocols represent a means of quickly adapting the inherent potential of this species to specific applications and markets. E. sativa has potential for food, feed, industrial, and medicinal purposes and is a pool of genetic diversity for the economically important Brassica species. Intergeneric hybrids with Brassica napus, B. rapa, and B. juncea have been produced through either embryo rescue or protoplast fusion. Brassica markers are transferrable to E. sativa making marker assisted selection possible. Tissue regeneration protocols focus around the use of cotyledons, hypocotyls or cotyledonary node explants. Low concentrations of \( \alpha \)-naphthaleneacetic acid (NAA) induce shoot organogenesis in 20 % of cultured explants, whereas somatic embryogenesis protocols focus around the use of 2, 4-dichlorophenoxyacetic acid (2, 4-D) and give a regeneration rate of greater than ~6 %. Double haploid protocols exist for both isolated microspore culture and anther culture. The key feature of these protocols is the need for heat shock to trigger androgenesis. An Agrobacterium tumefaciens-mediated transformation protocol based around shoot organogenesis gives a transformation frequency of 1.1 %.

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Abbreviations

2, 4-D:

2, 4-Dichlorophenoxyacetic acid

BA:

6-Benzyladenine

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butryic acid

NAA:

\( \alpha \)-Naphthaleneacetic acid

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  1. Crop Development Centre, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, S7N5A8, Canada

    Susan MH Slater

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  1. , Department of Agricultural, University of Helsinki, 5 Latokartanonkaari Room C 130, Helsinki, 00014, Finland

    Shri Mohan Jain

  2. Dept. Agricultural & Food Engineering, Indian Institute of Technology, Kharagpur, 721302, India

    S. Dutta Gupta

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Slater, S.M. (2013). Biotechnology of Eruca Sativa Mill. In: Jain, S., Dutta Gupta, S. (eds) Biotechnology of Neglected and Underutilized Crops. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-5500-0_9

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