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Natural and Artificial Proteins Containing Cadmium

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Cadmium: From Toxicity to Essentiality

Part of the book series: Metal Ions in Life Sciences ((MILS,volume 11))

Abstract

This chapter describes an approach using designed proteins to understand the structure, spectroscopy, and dynamics of proteins that bind Cd(II). We will show that three-stranded coiled coils (3SCCs) based on the parent peptides TRI (Ac-G(LKALEEK)4G-NH2) or GRAND (Ac-G(LKALEEK)5G-NH2) have been essential for understanding how Cd(II) binds to thiolate-rich environments in proteins. Examples are given correlating physical properties such as the binding constants or deprotonation constants relating to structure. We present a scale that relates 113Cd NMR chemical shifts to structures extracted from 111mCd PAC experiments. In addition, we describe motional processes that help transport from the helical interface of proteins into the hydrophobic interior of helical bundles. These studies help clarify the chemistry of Cd(II) in relation to metal-regulated gene expression and detoxification.

An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-94-007-5179-8_17

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Abbreviations

&:

For the definition of the peptides see Table 1.

Ala:

alanine

ALAD:

aminolevulinic acid dehydratase

Asp:

aspartic acid

CD:

circular dichroism

Cys:

cysteine

EXAFS:

extended X-ray absorption fine structure

Glu:

glutamic acid

Gly:

glycine

His:

histidine

Hfl:

hexafluoroleucine

Ile:

isoleucine

Leu:

leucine

LMCT:

ligand-to-metal charge-transfer

Met:

methionine

NOESY:

nuclear Overhauser effect specroscpy

PAC:

perturbed angular correlation

PDB:

Protein Data Bank

Pen:

penicillamine

NMR:

nuclear magnetic resonance

3SCC:

three-stranded coiled coil

Ser:

serine

Val:

valine

References

  1. T. Lane, M. A. Saito, G. N. George, I. J. Pickering, R. C. Prince, F. F. M. Morel, Nature 2005, 435, 42.

    Google Scholar 

  2. R. H. Holm, P. Kennepohl, E. I. Solomon, Chem. Rev. 1996, 96, 2239–2314.

    Article  PubMed  CAS  Google Scholar 

  3. M. Margoshes, B. L. Vallee, J. Am. Chem. Soc. 1957, 79, 4813–4814.

    Article  CAS  Google Scholar 

  4. L. M. Utschig, J. W. Bryson, T. V. O’Halloran, Science 1995, 268, 380–385.

    Article  PubMed  CAS  Google Scholar 

  5. D. M. Ralston, T. V. O’Halloran, Proc. Natl. Acad. Sci. USA 1990, 87, 3846–3850.

    Article  CAS  Google Scholar 

  6. R. P. Novick, C. Roth, J. Bacteriol. 1968, 95, 1335–1342.

    PubMed  CAS  Google Scholar 

  7. Y. Sun, M. Wong, B. P. Rosen, J. Biol. Chem. 2001, 276, 14955–14960.

    CAS  Google Scholar 

  8. C. Rensing, Y. Sun, B. Mitra, B. P. Rosen, J. Biol. Chem. 1998, 273, 32614–32617.

    CAS  Google Scholar 

  9. G. Endo, S. Silver, J. Bacteriol. 1995, 177, 4437–4441.

    PubMed  CAS  Google Scholar 

  10. J. Ye, A. Kandegedara, P. Martin, B. P. Rosen, J. Bacteriol. 2005, 187, 4214–4221.

    Article  PubMed  CAS  Google Scholar 

  11. A. Kandegedara, S. Thiyagarajann, K. C. Kondapalli, T. L. Stemmler, B. P. Rosen, J. Biol. Chem. 2009, 284, 14958–14965.

    CAS  Google Scholar 

  12. C. Rensing, J. Bacteriol. 2005, 187, 3909–3912.

    Article  PubMed  CAS  Google Scholar 

  13. L. S. Busenlehner, N. J. Cosper, R. A. Scott, B. P. Rosen, M. D. Wong, D. P. Giedroc, Biochemistry 2001, 40, 4426–4436.

    Article  PubMed  CAS  Google Scholar 

  14. L. S. Busenlehner, T.-C. Weng, J. E. Penner-Hahn, D. P. Giedroc, J. Mol. Biol. 2002, 319, 685–701.

    CAS  Google Scholar 

  15. M. F. Summers, Coord. Chem. Rev. 1988, 86, 43–134.

    Article  CAS  Google Scholar 

  16. A. K. Duhme, H. Z. Strasdeit, Z. Anorg. Allg. Chem. 1999, 625, 6–8.

    Article  CAS  Google Scholar 

  17. R. A. Santos, E. S. Gruff, S. A. Koch, J. Am. Chem. Soc. 1991, 113, 469–475.

    Article  CAS  Google Scholar 

  18. N. Govindaswamy, J. Moy, M. Millar, S. A. Koch, Inorg. Chem. 1992, 31, 5343–5344.

    Article  CAS  Google Scholar 

  19. J. S. Cavet, A. I. Graham, W. Meng, N. J. Robinson, J. Biol. Chem. 2003, 278, 44560–44566.

    CAS  Google Scholar 

  20. Y. Wang, L. Hemmingsen, D. P. Giedroc, Biochemistry 2005, 44, 8976–8988.

    Article  PubMed  CAS  Google Scholar 

  21. L. Banci, I. Bertini, F. Cantini, S. Ciofi-Baffoni, J. S. Cavet, C. Dennison, A. I. Graham, D. R. Harvie, N. J. Robinson, J. Biol. Chem. 2007, 282, 30181-30188.

    CAS  Google Scholar 

  22. B. Tripet, K. Wagschal, P. Lavigne, C. T. Mant, R. S. Hodges, J. Mol. Biol. 2000, 300, 377–402.

    CAS  Google Scholar 

  23. P. B. Harbury, T. Zhang, P. S. Kim, T. Albert, Science 1993, 262, 1401–1407.

    Article  PubMed  CAS  Google Scholar 

  24. B. Lovejoy, S. Choe, D. Cascio, D. K. McRorie, W. F. DeGrado, D. Eisenberg, Science 1993, 259, 1288–1293.

    Article  PubMed  CAS  Google Scholar 

  25. D. N. Woolfson, The Design of Coiled-Coil Structures and Assemblies, in Advances in Protein Chemistry, Vol. 70, Eds D. A. D. Parry, J. M. Squire, Academic Press, 2005, pp. 79–112.

    Google Scholar 

  26. O. D. Monera, C. M. Kay, R. S. Hodges, Biochemistry 1994, 33, 3862–3871.

    Article  PubMed  CAS  Google Scholar 

  27. E. K. O’Shea, K. J. Lumb, P. S. Kim, Curr. Biol. 1993, 3, 658–667.

    Article  PubMed  Google Scholar 

  28. O. Iranzo, D. Ghosh, V. L. Pecoraro, Inorg. Chem. 2006, 45, 9959–9973.

    Article  PubMed  CAS  Google Scholar 

  29. J. P. Schneide, J. W. Kelly, J. Am. Chem. Soc. 1995, 117, 2533–2546.

    Article  Google Scholar 

  30. G. Platt, C. Chung, M. Searle, Chem. Commun. 2001, 1162–1163.

    Google Scholar 

  31. J. Venkatraman, G. A. Naganagowda, R. Sudha, P. Balaram, Chem. Commun. 2001, 2660–2661.

    Google Scholar 

  32. R. P. Cheng, S. L. Fisher, B. J. Imperiali, J. Chem. Soc., Dalton Trans. 1996, 118, 11349–11356.

    Google Scholar 

  33. M. M. Rosenblatt, J. Y. Wang, K. S. Suslick, Proc. Natl. Acad. Sci. USA 2002, 100, 13140–13145.

    Article  Google Scholar 

  34. B. A. Krizek, D. L. Merkle, J. M. Berg, Inorg. Chem. 1993, 32, 937–940.

    Article  CAS  Google Scholar 

  35. M. D. Struthers, R. P. Cheng, B. J. Imperiali, J. Am. Chem. Soc. 1996, 118, 3073–3081.

    Article  CAS  Google Scholar 

  36. M. D. Struthers, R. P. Cheng, B. J. Imperiali, Science 1996, 271, 342–345.

    Article  PubMed  CAS  Google Scholar 

  37. R. P. Bonomo, L. Casella, L. D. Gioia, H. Molinari, G. Impellizzeri, T. Jordan, G. Pappalardo, E. Rizzarelli, J. Chem. Soc., Dalton Trans. 1997, 2387–2389.

    Google Scholar 

  38. G. R. Dieckmann, D. K. McRorie, J. D. Lear, K. A. Sharp, W. F. DeGrado, V. L. Pecoraro, J. Mol. Biol. 1998, 280, 897–912.

    CAS  Google Scholar 

  39. G. R. Dieckmann, D. K. McRorie, D. L. Tierney, L. M. Utschig, C. P. Singer, T. V. O’Halloran, J. E. Penner-Hahn, W. F. DeGrado, V. L. Pecoraro, J. Am. Chem. Soc. 1997, 119, 6195–6196.

    Article  CAS  Google Scholar 

  40. C. J. Reedy, B. R. Gibney, Chem. Rev. 2004, 104, 617–649.

    Article  PubMed  CAS  Google Scholar 

  41. B. R. Gibney, S. E. Mulholland, F. Rabanal, P. L. Dutton, Proc. Natl. Acad. Sci. USA 1996, 93, 15041–15046.

    Article  CAS  Google Scholar 

  42. V. L. Pecoraro, A. F. A. Peacock, O. Iranzo, M. Luczkowski, Understanding the biological chemistry of mercury using a de novo protein design strategy, in Bioinorg. Chem. ACS Sympos. Ser. 1012, , pp. 183–197.

    Google Scholar 

  43. B. T. Farrer, N. P. Harris, K. E. Balchus, V. L. Pecoraro, Biochem. 2001, 40, 14696–14705.

    Article  CAS  Google Scholar 

  44. B. T. Farrer, C. P. McClure, J. E. Penner-Hahn, V. L. Pecoraro, Inorg. Chem. 2000, 39, 5422–5423.

    Article  PubMed  CAS  Google Scholar 

  45. M. Matzapetakis, D. Ghosh, T.-C. Weng, J. E. Penner-Hahn, V. L. Pecoraro, J. Biol. Inorg. Chem. 2006, 11, 876–890.

    Article  PubMed  CAS  Google Scholar 

  46. B. T. Farrer, V. L. Pecoraro, Proc. Natl. Acad. Sci. USA 2003, 100, 3760–3765.

    Article  CAS  Google Scholar 

  47. O. Iranzo, P. W. Thulstrup, S. Ryu, L. Hemmingsen, V. L. Pecoraro, Chem. Eur. J. 2007, 13, 9178–9190.

    Article  PubMed  CAS  Google Scholar 

  48. D. S. Touw, C. E. Nordman, J. E. Stuckey, V. L. Pecoraro, Proc. Natl. Acad. Sci. USA 2007, 104, 11969–11974.

    Article  CAS  Google Scholar 

  49. M. Matzapetakis, B. T. Farrer, T.-C. Weng, L. Hemmingsen, J. E. Penner-Hahn, V. L. Pecoraro, J. Am. Chem. Soc. 2002, 124, 8042–8054.

    Article  PubMed  CAS  Google Scholar 

  50. M. Matzapetakis, V. L. Pecoraro, J. Am. Chem. Soc. 2005, 127, 18229–18233.

    Article  PubMed  CAS  Google Scholar 

  51. N. Ogihara, M. S. Weiss, W. F. DeGrado, D. Eisenberg, Protein Sci. 1997, 6, 78–86.

    Google Scholar 

  52. K. T. O’Neil, W. F. DeGrado, Science 1990, 250, 646–651.

    Article  PubMed  Google Scholar 

  53. S. Chakraborty, D. S. Touw, A. F. A. Peacock, J. Stuckey, V. L. Pecoraro, J. Am. Chem. Soc. 2010,132, 13240–13250.

    Article  PubMed  CAS  Google Scholar 

  54. L. Hemmingsen, K. Nárcisz, E. Danielsen, Chem. Rev. 2004, 104, 4027–4061.

    Article  PubMed  CAS  Google Scholar 

  55. L. Hemmingsen, L. Olsen, J. Antony, S. P. A. Sauer, J. Biol. Inorg. Chem. 2004, 9, 591–599.

    Article  PubMed  CAS  Google Scholar 

  56. J. C. L. Reynolds, K. F. Cooke, S. H. Northrup, J. Phys. Chem. 1990, 94, 985–991.

    CAS  Google Scholar 

  57. T. L. Poulos, B. C. Finzel, A. J. Howard, Biochemistry 1986, 25, 5314–5322.

    Article  PubMed  CAS  Google Scholar 

  58. O. D. Monera, F. D. Sönnichsen, L. Hicks, C. M. Kay, R. S. Hodges, Protein Eng. 1996, 9, 353–363.

    Article  PubMed  CAS  Google Scholar 

  59. K.-H. Lee, M. Matzapetakis, S. Mitra, E. N. G. Marsh, V. L. Pecoraro, J. Am. Chem. Soc. 2004, 126, 9178–9179.

    Article  PubMed  CAS  Google Scholar 

  60. K.-H. Lee, C. Cabello, L. Hemmingsen, E. N. G. Marsh, V. L. Pecoraro, Angew. Chem. Int. Ed. 2006, 45, 2864–2868.

    Article  CAS  Google Scholar 

  61. C. N. Pace, J. M. Scholtz, Biophys. J. 1998, 75, 422–427.

    Article  PubMed  CAS  Google Scholar 

  62. P. T. Erskine, E. M. H. Duke, I. J. Tickle, N. M. Senior, M. J. Warren, J. B. Cooper, Acta Crystallogr. Sect. D 2000, 56, 421–430.

    Article  CAS  Google Scholar 

  63. O. Iranzo, T. Jakusch, K.H. Lee, L. Hemmingsen, V. L. Pecoraro, Chem. Eur. J. 2009, 15, 3761–3772.

    Article  PubMed  CAS  Google Scholar 

  64. A. F. A. Peacock. J. A. Stuckey, V. L. Pecoraro, Angew. Chem. Int. Ed. 2009, 48, 7371–7374.

    Google Scholar 

  65. A. F. A. Peacock, L. Hemmingsen, V. L. Pecoraro, Proc. Natl. Acad. Sci. USA 2008, 105, 16566–16571.

    Article  CAS  Google Scholar 

  66. M. Łuczkowski, M. Stachura, V. Schirf, B. Demeler, L. Hemmingsen, V. L. Pecoraro, Inorg. Chem. 2008, 47, 10875–10888.

    Article  PubMed  Google Scholar 

  67. O. A. Kharenko, M. Y. Ogawa, J. Inorg. Biochem. 2004, 98, 1971–1974.

    CAS  Google Scholar 

  68. O. A. Kharenko, D. C. Kennedy, B. Demeler, M. J. Maroney, M. Y. Ogawa, J. Am. Chem. Soc. 2005, 127, 7678–7679.

    Article  PubMed  CAS  Google Scholar 

  69. M. Mukherjee, X. Zhu, M. Y. Ogawa, Inorg. Chem. 2008, 47, 4430–4432.

    Article  PubMed  CAS  Google Scholar 

  70. C. J. Henehan, D. L. Pountney, O. Zerbe, M. Vašák, Protein Sci. 1993, 2, 1756–1764.

    Article  PubMed  CAS  Google Scholar 

  71. D. S. Touw, Ph.D. Thesis, University of Michigan, 2007.

    Google Scholar 

  72. D. Ghosh, K.-H. Lee, B. Demeler, V. L. Pecoraro, Biochemistry 2005, 44, 10732–10740.

    Article  PubMed  CAS  Google Scholar 

  73. J. Y. Su, R. S. Hodges, C. M. Kay, Biochemistry 1994, 33, 15501–15510.

    Article  PubMed  CAS  Google Scholar 

  74. O. Iranzo, C. Cabello, V. L. Pecoraro, V. L., Angew. Chem. Int. Ed. 2007, 46, 6688–6691.

    Google Scholar 

  75. O. Iranzo, S. Chakraborty, L. Hemmingsen, V. L. Pecoraro, J. Am. Chem. Soc. 2011, 133, 239–251.

    Article  PubMed  CAS  Google Scholar 

  76. S. T. R. Walsh, H. Cheng, J. W. Bryson, H. Roder, W. F. DeGrado, Proc. Natl. Acad. Sci. USA 1999, 96, 5486–5491.

    Article  CAS  Google Scholar 

  77. S. Chakraborty, J. Yudenfreund Kravitz, P. W. Thulstrup, L. Hemmingsen, W. F. DeGrado, V. L. Pecoraro, Angew. Chem. Int. Ed. 2011, 50, 2049–2053.

    Article  CAS  Google Scholar 

  78. R. K. Bogess, J. R. Absher, S. Morelen, L. T. Taylor, J. W. Hughes, Inorg. Chem. 1983, 22, 1273–1279.

    Article  Google Scholar 

  79. B. V. Cheesman, A. P. Arnold, D. L. Rabenstein, J. Am. Chem. Soc. 1988, 110, 6359–6364.

    Article  CAS  Google Scholar 

  80. S. Chakraborty, O. Iranzo, E. R. P. Zuiderweg, V. L. Pecoraro, J. Am.Chem. Soc. 2012, 134, 6191–6203.

    Google Scholar 

  81. M. Zastrow, A. F. A. Peacock, J. Stuckey, V. L. Pecoraro, Nature Chem., 2012, 4, 118–123.

    Article  CAS  Google Scholar 

  82. A. F. A. Peacock, O. Iranzo, V. L. Pecoraro, Dalton Trans. 2009, 13, 2271–2280.

    Article  PubMed  Google Scholar 

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Acknowledgments

A.F.A.P. thanks the University of Birmingham and V.L.P. thanks the University of Michigan and the National Institute of Health for support of this research (R01 ES0 12236).

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Authors and Affiliations

  1. Department of Chemistry, University of Michigan, Ann Arbor, Michigan, 48109, USA

    Anna F. A. Peacock

  2. School of Chemistry, University of Birmingham, Edgbaston, B15 2TT, UK

    Vincent L. Pecoraro

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  1. Anna F. A. Peacock
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Correspondence to Anna F. A. Peacock or Vincent L. Pecoraro .

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Editors and Affiliations

  1. Inorganic Chemistry, Chemistry, Universität Basel, Spitalstr. 51, Basel, 4056, Basel Stadt, Switzerland

    Astrid Sigel

  2. Inst. Anorganische Chemie, Universität Basel, Spitalstr. 51, Basel, 4056, Switzerland

    Helmut Sigel

  3. Institute of Inorganic Chemistry, University of Zürich, Winterthurerstrasse 190, Zürich, 8057, Zürich, Switzerland

    Roland KO Sigel

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Peacock, A.F.A., Pecoraro, V.L. (2013). Natural and Artificial Proteins Containing Cadmium. In: Sigel, A., Sigel, H., Sigel, R. (eds) Cadmium: From Toxicity to Essentiality. Metal Ions in Life Sciences, vol 11. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-5179-8_10

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