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MicroRNAs as Tools in Biopharmaceutical Production pp 1–13Cite as

A General Introduction to MicroRNAs, Their Investigation and Exploitation in CHO Cell Lines

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Abstract

MicroRNAs are small (20–23 nucleotides) non-coding RNA molecules that mainly act as negative regulators of gene expression by binding to target mRNAs in their 3’UTR. In this chapter, we provide a brief account of the in silico and experimental tools available for researchers working in protein engineering in eukaryotic cell factories. In considering the wide influence of microRNAs, we place a special emphasis on the cellular effects associated with the manipulation of microRNA and high yield of protein production with reference to use in Chinese hamster ovary (CHO) expression systems.

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Authors and Affiliations

  1. Centre for Molecular Processing and School of Biosciences, University of Kent, CT2 7NJ, Canterbury, Kent, UK

    Lyne Jossé & C Mark Smales

  2. Pfizer Inc, 1 Burtt Road, 01810, Andover, MA, USA

    Lin Zhang

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  1. Lyne Jossé
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  2. Lin Zhang
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  3. C Mark Smales
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Correspondence to Lyne Jossé .

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  1. , National Institute for Cellular Biotechn, Dublin City University, Dublin, Ireland

    Niall Barron

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Jossé, L., Zhang, L., Smales, C. (2012). A General Introduction to MicroRNAs, Their Investigation and Exploitation in CHO Cell Lines. In: Barron, N. (eds) MicroRNAs as Tools in Biopharmaceutical Production. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-5128-6_1

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