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Electric Cell-Substrate Impedance Sensing and Cancer Metastasis pp 217–237Cite as

ECIS, Cellular Adhesion and Migration in Keratinocytes

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Part of the book series: Cancer Metastasis - Biology and Treatment ((CMBT,volume 17))

Abstract

Evaluation of keratinocyte adhesion and migration using ECIS, as will be shown below, is predominantly used to study the effects of various genes, proteins and molecules on wound healing. Keratinocytes undertake epithelialisation during the process of wound healing, and understanding their function, and their regulating factors, is important in understanding why some wounds fail to heal. This chapter will detail the physiology behind wound healing and epithelialisation, and explore the problem that chronic wounds pose to the clinician today. It will also highlight the importance of both adhesion and migration in cellular movements. Furthermore it will detail the methods by which ECIS has been used to evaluate keratinocyte function, and discuss various methods of interpreting ECIS results. Finally, it will review the current literature on ECIS evaluation of keratinocyte function.

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Abbreviations

AC:

Alternating Current

ALCAM:

Activated Leukocyte Cell Adhesion Molecule

BMP7:

Bone Morphogenetic Protein 7

CWIS:

Cardiff Wound Impact Schedule

DC:

Direct Current

DM:

Diabetes Mellitus

DVT:

Deep Vein Thrombosis

ECIS:

Electrical Cell-Substrate Impedance Sensing

ECM:

Extra Cellular Matrix

Ehm2:

Expressed in high metastatic cells 2

EPLIN:

Epithelial Protein Lost In Neoplasm

FAs:

Focal Adhesions

HRQoL:

Health-related quality of life

HuR:

Human antigen R

IL8:

Interleukin 8

MDA7:

Melanoma Differentiation Associated gene 7

NPFs:

Nucleation-Promoting Factors

PAD:

Peripheral Arterial Disease

PIP2:

Phosphatidylinositol 4,5-bisphosphate

TEM8:

Tumour Endothelial Marker 8

WAVE:

Wiskott–Aldrich syndrome protein family VErprolin homologs

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Authors and Affiliations

  1. Departments of Wound Healing and Surgery, Cardiff University School of Medicine, Heath Park, Cardiff, CF14 4XN, UK

    David C. Bosanquet, Keith G. Harding & Wen G. Jiang

Authors
  1. David C. Bosanquet
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  2. Keith G. Harding
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  3. Wen G. Jiang
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Corresponding author

Correspondence to David C. Bosanquet .

Editor information

Editors and Affiliations

  1. Research Group Dept. Of Surgery College, Of Medicine, Univ.Of Wales Metastatis, Heath Park, Cardiff, CF4 4XN, United Kingdom

    Wen G. Jiang

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Bosanquet, D.C., Harding, K.G., Jiang, W.G. (2012). ECIS, Cellular Adhesion and Migration in Keratinocytes. In: Jiang, W. (eds) Electric Cell-Substrate Impedance Sensing and Cancer Metastasis. Cancer Metastasis - Biology and Treatment, vol 17. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4927-6_12

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