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Electric Cell-Substrate Impedance Sensing as a Screening Tool for Wound Healing Agents

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Electric Cell-Substrate Impedance Sensing and Cancer Metastasis

Part of the book series: Cancer Metastasis - Biology and Treatment ((CMBT,volume 17))

Abstract

As one of the earliest matured techniques for in vitro cell migration study in wound healing, traditional scratch assay has been routinely utilized due to its simplicity of setup in cell culture. However, with the emerging needs of acquiring high sensitivity and achieving high-throughput in cell behavior study, researchers in life science started to apply electric cell-substrate impedance sensing (ECIS) technology, with its automated real-time impedance monitoring and standard electrical wounding, in various study fields. Nonetheless, not much information is available regarding the application of ECIS in the screening of wound healing agents. Here we first reviewed the applications of both traditional scratch assay and ECIS model in wound healing. We further used a herbal formula NF3 (comprising of individual herbs Astragali Radix and Rehmanniae Radix in the ratio of 2:1) which was previously shown to exhibit profound wound-healing effect in diabetic foot ulcer rat model, as an example for comparison of cell migration studies using both traditional scratch assay and ECIS model. To conclude, with its high sensitivity and efficiency, ECIS demonstrated its reliability as a tool for the screening of wound healing agents.

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Acknowledgement

This study was supported by the University Grants Committee of the Hong Kong SAR under the Area of Excellence project “Chinese Medicine Research and Further Development” (Ref. No. AoE/B-10/01). We would also like to thank Hop Wai short-term research fellowship for the sponsorship of ECIS training in Cardiff University School of Medicine, Cardiff, United Kingdom.

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Authors and Affiliations

  1. Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong

    Cheuk Lun Liu, Jacqueline Chor Wing Tam, Chun Hay Ko, Kwok Pui Fung, Ping Chung Leung & Clara Bik San Lau

  2. State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong

    Cheuk Lun Liu, Jacqueline Chor Wing Tam, Chun Hay Ko, Kwok Pui Fung, Ping Chung Leung & Clara Bik San Lau

  3. School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong

    Cheuk Lun Liu & Kwok Pui Fung

  4. Metastasis and Angiogenesis Research Group, Cardiff University School of Medicine, Cardiff, UK

    Andrew J. Sanders, David G. Jiang & Wen G. Jiang

  5. Department of Dermatology and Wound Healing, Cardiff University School of Medicine, Cardiff, UK

    Keith G. Harding

Authors
  1. Cheuk Lun Liu
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  2. Jacqueline Chor Wing Tam
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  3. Andrew J. Sanders
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  4. David G. Jiang
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  5. Chun Hay Ko
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  6. Kwok Pui Fung
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  7. Ping Chung Leung
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  8. Keith G. Harding
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  9. Wen G. Jiang
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  10. Clara Bik San Lau
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Corresponding authors

Correspondence to Wen G. Jiang or Clara Bik San Lau .

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Editors and Affiliations

  1. Research Group Dept. Of Surgery College, Of Medicine, Univ.Of Wales Metastatis, Heath Park, Cardiff, CF4 4XN, United Kingdom

    Wen G. Jiang

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© 2012 Springer Science+Business Media Dordrecht

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Liu, C.L. et al. (2012). Electric Cell-Substrate Impedance Sensing as a Screening Tool for Wound Healing Agents. In: Jiang, W. (eds) Electric Cell-Substrate Impedance Sensing and Cancer Metastasis. Cancer Metastasis - Biology and Treatment, vol 17. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4927-6_11

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