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Alpha-Amylase Allelic Variation in Domesticated and Wild Barley

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Abstract

Barley germplasm including breeding lines, wild barley accessions and mapping population parents were screened for variation in α-amylase using isoelectric focusing (IEF) in conjunction with activity staining. IEF screening found extensive variation in α-amylase IEF banding patterns with 14 and 49 different α-amylase isoenzymes identified for low- and high-pI groups, respectively. Alpha-amylase enzyme activity assayed at a range of temperatures (40–75 °C) revealed significant variation in the levels of enzyme activity and thermostability between varieties belonging to different IEF groups. The relationship between fermentability and α-amylase activity and thermostability was studied in 30 elite breeding lines and variation in thermostability between IEF groups was found to have a significant impact on fermentability.

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Acknowledgement

The authors would like to acknowledge GRDC for funding this project (UA00108).

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Authors and Affiliations

  1. School of Agriculture Food and Wine, The University of Adelaide, Waite Campus, Glen Osmond, Adelaide, South Australia, 5064, Australia

    Suong Cu, Sophia Roumeliotis & Jason Eglinton

Authors
  1. Suong Cu
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  2. Sophia Roumeliotis
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  3. Jason Eglinton
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Corresponding author

Correspondence to Suong Cu .

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Editors and Affiliations

  1. Agronomy Department, Zhejiang University, Hangzhou, 310029, China, People's Republic

    Guoping Zhang

  2. Dept. Agriculture Western Australia, Government of Western Australia, Baron Hay-Court 3, South Perth, 6151, West Australia, Australia

    Chengdao Li

  3. , Institute of Crop Science, Chinese Academy of Agricultural Sciences, 12 South Street, Zhongguancun, Beijing, 100081, China, People's Republic

    Xu Liu

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© 2013 Zhejiang University Press and Springer Science+Business Media Dordrecht

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Cu, S., Roumeliotis, S., Eglinton, J. (2013). Alpha-Amylase Allelic Variation in Domesticated and Wild Barley. In: Zhang, G., Li, C., Liu, X. (eds) Advance in Barley Sciences. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4682-4_5

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