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Site-Directed Insertion of Transgenes in Drosophila

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Part of the book series: Topics in Current Genetics ((TCG,volume 23))

Abstract

The fruit fly, Drosophila melanogaster, has been a popular and productive model organism for more than a century. Methods for essentially random transgene introduction developed in the 1980s have been supplemented more recently with site-specific and targeted approaches. Integrated recognition sites for PhiC31 integrase allow reproducible locations for related transgenes. Targeting through homologous recombination has been achieved by in situ generation of linear donor DNA. Enhanced targeting frequencies have been achieved by making a double-strand break in the target with zinc-finger nucleases (ZFNs). Relative strengths and weaknesses of these approaches are considered.

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Correspondence to Dana Carroll .

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Carroll, D. (2013). Site-Directed Insertion of Transgenes in Drosophila . In: Renault, S., Duchateau, P. (eds) Site-directed insertion of transgenes. Topics in Current Genetics, vol 23. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4531-5_12

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