Abstract
Embryonic stem (ES) cells have been investigated in many animal models of severe injury and degenerative disease. However, few studies have examined the ability of ES cells to improve functional outcome following mammary gland injury. This study investigates the feasibility of implanting mouse ES cells labeled with enhanced green fluorescence protein in the developing mammary glands in order to acquire lineage-committed cells in mammary (mammary gland epithelial cell or luminal cell). Cells implanted in high numbers (5 × 106 cells per mammary gland) survived in the majority of the mice and nearly 38.4% of the surviving cells were CK18+ at 15th week following the transplantation. These results maybe provide a technique instrument on advanced therapy of breast diseases and the mammary regeneration after breast ablated partly.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Altschuler RA, O’Shea KS, Miller JM (2008) Stem cell transplantation for auditory nerve replacement. Hear Res 242:110–116
Alvi AJ, Clayton H, Joshi C, Enver T, Ashworth A, Vivanco MM, Dale TC, Smalley MJ (2003) Functional and molecular characterisation of mammary side population cells. Breast Cancer Res 5:R1–R8
Becerra GD, Tatko LM, Pak ES, Murashov AK, Hoane MR (2007) Transplantation of GABAergic neurons but not astrocytes induces recovery of sensorimotor function in the traumatically injured brain. Behav Brain Res 179:118–125
Bjorklund LM, Sánchez-Pernaute R, Chung S, Andersson T, Chen LYC, McNaught KSP (2002) Embryonic stem cells develop into functional dopaminergic neurons after transplantation in a Parkinson rat model. Proc Natl Acad Sci USA 99:2344–2349
Booth BW, Boulanger CA, Smith GH (2008a) Stem cells and the mammary microenvironment. Breast Dis 29:57–67
Booth BW, Mack DL, Androutsellis-Theotokis A, McKay RD, Boulanger CA, Smith GH (2008b) The mammary microenvironment alters the differentiation repertoire of neural stem cells. Proc Natl Acad Sci USA 105:14891–14896
Boulanger CA, Smith GH (2009) Reprogramming cell fates in the mammary microenvironment. Cell Cycle 8:1127–1132
Boulanger CA, Mack DL, Booth BW, Smith GH (2007) Interaction with the mammary microenvironment redirects spermatogenic cell fate in vivo. Proc Natl Acad Sci USA 104:3871–3876
DeOme KB, Faulkin LJ, Bern HA, Blair PB (1959) Development of mammary tumors from hyperplastic alveolar nodules transplanted into gland free mammary fat pads of female C3H mice. Cancer Res 19:512–520
Evans MJ, Kaufman MH (1981) Establishment in culture of pluripotential cells from mouse embryos. Nature 292:154–156
Forsyth IA (1986) Variation among species in the endocrine control of mammary growth and function: the roles of prolactin, growth hormone and placental lactogen. J Dairy Sci 69:886–903
Kim D, Dressler GR (2005) Nephrogenic factors promote differentiation of mouse embryonic stem cells into renal epithelia. J Am Soc Nephrol 16:3527–3534
Kordon EC, Smith GH (1998) An entire functional mammary gland may comprise the progeny from a single cell. Development 125:1921–1930
Kudo K, Abe Y, Hu DL, Kijima H, Nakane A (2007) Colonization and differentiation of transplanted embryonic stem cells in the irradiated intestine of mice. Tohoku J Exp Med 212:143–150
Motohashi T, Aoki H, Yoshimura N, Kunisada T (2006) Induction of melanocytes from embryonic stem cells and their therapeutic potential. Pigment Cell Res 19:284–289
Nagy A, Gertsenstein M, Vintersten K, Behringer RR (2003) In: Nagy A, Gertsenstein M (eds) Isolation and culture of blastocyst-derived stem cell lines. Manipulating the mouse embryo: a laboratory manual. Cold Spring Harbor Laboratory Press, New York, pp 453–506
Regala C, Duan M, Zou J, Salminen M, Olivius P (2005) Xenografted fetal dorsal root ganglion, embryonic stem cell and adult neural stem cell survival following implantation into the adult vestibulocochlear nerve. Exp Neurol 193:326–333
Rijnkels M, Rosen JM (2001) Adenovirus-Cre-mediated recombination in mammary epithelial early progenitor cells. J Cell Sci 114:3147–3153
Robinson AJ, Meedeniya AC, Hemsley KM (2005) Survival and engraftment of mouse embryonic stem cell-derived implants in the guinea pig brain. Neurosci Res 53:161–168
Shackleton M, Vaillant F, Simpson KJ, Stingl J, Smyth GK, Asselin-Labat ML, Wu L, Lindeman GJ, Visvader JE (2006) Generation of a functional mammary gland from a single stem cell. Nature 439:84–88
Smith GH, Gallahan D, Zwiebel JA, Freeman SM, Bassin RH, Callahan R (1991) Long-term in vivo expression of genes introduced by retrovirus-mediated transfer into mammary epithelial cells. J Virol 65:6365–6370
Srivastava AS, Shenouda S, Mishra R, Carrier E (2006) Transplanted embryonic stem cells successfully survive, proliferate, and migrate to damaged regions of the mouse brain. Stem Cells 24:1689–1694
Topper YJ, Freeman CS (1980) Multiple hormone interactions in the developmental biology of the mammary gland. Physiol Rev 60:1049–1056
Weiss ML, Mitchell KE, Hix JE, Medicetty S, El-Zarkouny SZ, Greiger D, Troyer DL (2003) Transplantation of porcine umbilical cord matrix cells into the rat brain. Exp Neurol 182:288–299
Welm BE, Tepera SB, Venezia T, Graubert TA, Rosen JM, Goodell MA (2002) Sca-l (pos) cells in the mouse mammary gland represent an enriched progenitor cell population. Dev Biol 245:42–56
Yang W, Hayward SW, Cao M, Thayer KA, Cunha GR (2001) Cell differentiation lineage in the prostate. Differentiation 68:270–279
Zhou QJ, Huang YD, Xiang LX, Shao JZ, Zhou GS, Yao H, Dai LC, Lu YL (2007) In vitro differentiation of embryonic stem cells into hepatocytes induced by fibroblast growth factors and bone morphological protein-4. Int J Biochem Cell Biol 39:1714–1721
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Huang, HJ., Gao, QS., Qian, YG., Li, J. (2012). Mammary Regeneration Using Embryonic Stem Cell Engraftment. In: Hayat, M. (eds) Stem Cells and Cancer Stem Cells, Volume 7. Stem Cells and Cancer Stem Cells, vol 7. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4285-7_7
Download citation
DOI: https://doi.org/10.1007/978-94-007-4285-7_7
Published:
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-007-4284-0
Online ISBN: 978-94-007-4285-7
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)