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Propagation of Human Bone Marrow Stem Cells for Craniofacial Applications

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Stem Cells and Cancer Stem Cells, Volume 7

Part of the book series: Stem Cells and Cancer Stem Cells ((STEM,volume 7))

Abstract

Cleft lip and/or palate is the second most frequent congenital defect in humans. Patients with this defect are treated by multi-disciplinary teams, which provide corrective procedures to improve aesthetic and function, as well as permanent psychosocial support. Frequently, these patients have corrective surgical procedures conducted to repair bone defects using different bone sources. Generally, modern surgical results are successful, although they can be improved in some aspects such as their reliability, especially for growing children. Novel combined approaches based on tissue engineering and Mesenchymal Stem Cells (MSCs) have been proposed. This chapter is mainly focused on the collection and ex-vivo propagation of hBMSCs/osteoprogenitors and their behavior in engineered microenvironments for craniofacial applications. Different aspects of isolation, propagation and osteoblastic phenotype expression are described. Also, the microfabrication of microenvironments and their effects on molecular and cellular components are explained.

These authors contributed equally to this work

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Acknowledgments

The authors acknowledge the current and former members of Professor Fernandes group in the Faculty of Dental Medicine, Professor Monteiro group in the Instituto de Engenharia Biomedica and the Faculty of Engineering of University of Porto, (Porto, Portugal), Professor Hansford group the Biomedical Engineering Department of The Ohio State University (Columbus, OH, USA). This work was supported partially by the Portuguese Science and Technology Foundation (Scholarship FCT/SFRH/BD/36220/2007 and Grant No.FCT/PTDC/CTM/100120/2008 “Bonamidi”), CRUP – Acções integradas Luso-Espanholas: E46/09, Acciones integradas Hispano-Portuguesas, MICINN: HP2008-0075. NSF Nanoscale Science and Engineering Center fellowship (NSF, Grant No.EEC-0425626), and an U.S. Air Force Office of Scientific Research MURI (Grant No.F49620-03-1-0421). DFG is supported by the Faculty of Dentistry at Universidad de Antioquia and the Faculty of Dentistry at CES University (Medellin, Colombia), and Rush University (Chicago, IL, USA) and Fulbright Grant “Profesor Universitario en Antioquia – 2010”.

Author information

Authors and Affiliations

  1. INEB – Instituto de Nacional de Engenharia Biomédica, Porto, Portugal

    Alejandro Pelaez-Vargas & Fernando J. Monteiro

  2. Universidade do Porto, Porto, Portugal

    Alejandro Pelaez-Vargas

  3. Facultad de Odontología, Universidad Cooperativa de Colombia, Medellín, Colombia

    Alejandro Pelaez-Vargas

  4. Biomedical Engineering Department, Nanoscale Science and Engineering Center, Ohio Nanotech West Laboratory, The Ohio State University, Columbus, OH, USA

    Daniel Gallego-Perez & Derek J. Hansford

  5. Rush University, Universidad de Antioquia and Universidad CES, Chicago, Il, USA

    David F. Gomez

  6. Medellín, Colombia

    David F. Gomez

  7. Faculdade de Medicina Dentária, Universidade do Porto, Porto, Portugal

    Maria H. Fernandes

  8. Faculdade de Engenharia – DEMM, Universidade do Porto, Porto, Portugal

    Fernando J. Monteiro

Authors
  1. Alejandro Pelaez-Vargas
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  2. Daniel Gallego-Perez
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  3. David F. Gomez
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  4. Maria H. Fernandes
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  5. Derek J. Hansford
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  6. Fernando J. Monteiro
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Corresponding authors

Correspondence to Alejandro Pelaez-Vargas or Daniel Gallego-Perez .

Editor information

Editors and Affiliations

  1. Biology, Kean University, Union, New Jersey, USA

    M.A. Hayat

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Pelaez-Vargas, A., Gallego-Perez, D., Gomez, D.F., Fernandes, M.H., Hansford, D.J., Monteiro, F.J. (2012). Propagation of Human Bone Marrow Stem Cells for Craniofacial Applications. In: Hayat, M. (eds) Stem Cells and Cancer Stem Cells, Volume 7. Stem Cells and Cancer Stem Cells, vol 7. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4285-7_10

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