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The Reduction of Callus Formation During Bone Regeneration by BMP-2 and Human Adipose Derived Stem Cells

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Stem Cells and Human Diseases

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Abstract

Reconstructive medicine, trauma surgery and orthopaedics show an enormous increase in numbers of patients with degenerative diseases. Thus the demand for new therapeutic approaches is continuously growing. New technologies, the so called “Tissue Engineering” offers with the combination of the three components: cells, growth factor and matrix, new promising technologies.

In our studies, a 2 mm transcortical non-critical size drill hole in the middle of the femur shaft of male rats was applied as a small defect model that was used as a screening model for bone regeneration as well as the in vivo bone healing stimulation when the growth factor BMP-2 was embedded together with ASCs in a locally-applied fibrin matrix. After relatively short periods of times (2 and 4 weeks) our small animal model demonstrated that it is possible to get information about the osteogenetic potential and bone regeneration with little effort (no osteosynthesis). The most significant result of our scientific project with the help of micro-computer tomography and descriptive histology analysis is the fact that the combination of ASCs + BMP-2 in a fibrin matrix significantly reduces the callus reaction after 2 weeks. ASCs embedded alone in the fibrin matrix did not cause an increased bone regeneration. Consequently these stem cells rather prevented the osteoinductive reaction of BMP-2 and thereby less callus formation could be analysed.

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Acknowledgement

We thank our colleagues Karin Hahn, Christoph Castellani, Asmita Banerjee, Daniela Dopler, Tatjana Morton, Martina Moritz, Susanne Wolbank for their support during the completion of the project as well as Gerald Zanoni and Stefan Tangl.

We also want to express our gratefulness to Monika Großauer and Mohammad Jafamadar for their excellent help during the preparations of the book chapter, Ilse Jung for statistically analysing our data.

This work was carried out under the scope of the European NoE EXPERTISSUES (NMP3-CT-2004-500283).

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Authors and Affiliations

  1. Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Research Center of the Austrian Worker’s Compensation Board, Austrian Cluster for Tissue Regeneration, Donaueschingenstrasse 13, A-1200, Vienna, Austria

    Claudia Keibl DVM & Martijn van Griensven

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  1. Claudia Keibl DVM
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  2. Martijn van Griensven
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Correspondence to Claudia Keibl DVM .

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Editors and Affiliations

  1. , Pharmacology and Toxicology, University of Kansas Medical Center, Rainbow Blvd 3901, Kansas City, 66160, Kansas, USA

    Rakesh Srivastava

  2. , Pathology and Laboratory Medicine, University of Kansas Medical Center, Rainbow Blvd 3901, Kansas City, 66160, Kansas, USA

    Sharmila Shankar

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Keibl, C., van Griensven, M. (2012). The Reduction of Callus Formation During Bone Regeneration by BMP-2 and Human Adipose Derived Stem Cells. In: Srivastava, R., Shankar, S. (eds) Stem Cells and Human Diseases. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-2801-1_12

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