Abstract
Generation of induced pluripotent stem (iPS) cells have the potential for investigation of molecular mechanisms of disease, understanding the development of human tissues, and clinical applications. Although it was reported that various kinds of human somatic cells reprogrammed into iPS cells, most of them showed low reprogramming efficiency. Previously, we reported that clonally-expanded mesenchymal stromal/stem cells (MSCs) derived from tooth germs of human third molars efficiently reprogrammed into iPS cells by retroviral transduction of OCT3/4, SOX2, and KLF4 without c-MYC. Our data indicated that human third molars, which usually discarded as clinical wastes, are a valuable cell source for generation of iPS cells. Here, we describe detailed methods of the MSCs preparation and iPS cells generation from the MSCs.
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Oda, Y., Ohnishi, H., Yuba, S., Ohgushi, H. (2012). Generation of Induced Pluripotent Stem Cells from Mesenchymal Stromal Cells Derived from Human Third Molars (Method). In: Hayat, M. (eds) Stem Cells and Cancer Stem Cells, Volume 2. Stem Cells and Cancer Stem Cells, vol 2. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-2016-9_9
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DOI: https://doi.org/10.1007/978-94-007-2016-9_9
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