Abstract
The well-characterized virulence factors of Yersinia pestis the causative agent of plague are those encoded by the virulence plasmids. Previously we have isolated an attenuated Y. pestis transposon insertion mutant in which the chromosomal pcm gene was disrupted. The pcm gene is located within a putative stress response locus that includes the surE, nlpD, and rpoS genes. In this study, we investigated the expression and the role of pcm locus genes in Y. pestis pathogenesis by constructing a set of isogenic surE, pcm, nlpD and rpoS mutants in the fully virulent Kimberley53 strain. We show that the NlpD lipoprotein is the only factor encoded from the pcm locus that is essential for Y. pestis virulence. A chromosomal deletion of the nlpD gene sequence resulted in a drastic reduction in virulence to an LD50 of at least 107 cfu for subcutaneous and airway routes of infection. The mutant was unable to colonize mouse organs following infection. The unsegmented morphology of the nlpD mutant indicates that NlpD is involved in cell separation; however, deletion of nlpD did not affect in vitro growth rate. Trans-complementation experiments with the Y. pestis nlpD gene restored virulence and all other phenotypic defects. Finally, we demonstrate that the nlpD mutant could be used as a very potent live vaccine against bubonic and pneumonic plague.
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Acknowledgments
We thank Mrs. Brut P. and Mrs. Stein D. for excellent technical assistance. We also thank Dr. François Norel from the Institute Pasteur Paris, France for the generous gift of RpoS antibodies.
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Tidhar, A. et al. (2010). The NlpD Lipoprotein of Yersinia pestis is Essential for Cell Separation and Virulence. In: Shafferman, A., Ordentlich, A., Velan, B. (eds) The Challenge of Highly Pathogenic Microorganisms. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9054-6_6
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DOI: https://doi.org/10.1007/978-90-481-9054-6_6
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