Abstract
We developed that induced with a sodium butyrate mass production of cell-size vesicles from several human lymphoma cells. Ramos, a human burkitt lymphoma was the best induction rate among several human cell lines. However, all cells were dead after NaB treatment. The trypan blue dye staining showed the leakless of the vesicle. Therefore, it is suggested that the vesicle could be available for a drug carrier. The immunostaining analysis showed the retainment of surface antigen such as IgM, implying the possibility of specific vesicle production by transfection of desired gene into viable cells followed by the NaB treatment. This study is applicable to membrane protein isolation, drug delivery system, and microreactor development.
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Kawahara, H., Shirahata, S., Tachibana, H., and Murakami, H. (1992) In vitro immunization of human lymphocytes with human lung cancer cell line A549. Hum. Antibod. Hybridomas 3: 8–13.
Suzuki, R., Oda, Y., Utoguchi, N., Kadowaki, N., and Maruyama, K. (2008) Development of novel antigen delivery system to dendritic cells using liposome technology in cancer immunotherapy. Drug Delivery Sys. 23(2): 123–129.
Noireaux, V., Libchaber, A., et al. (2004) A vesicle bioreactor as a step toward an artifical cell assembly. Proc. Natl. Acad. Sci. USA 101:17669–17674.
Rigaud, J.L. and Levy D. (2003) Reconstitution of Membrane Proteins into Liposomes. Method. Enzymol. 372: 65–86.
Vidali, G., Boffa, L.C., Bradbury, E.M., and Allfrey, V.G. (1978) Butyrate suppression of histone deacetylation leads to accumulation of multiacetylated forms of histones H3 and H4 and increased DNase I sensitivity of the associated DNA sequences. Proc. Natl. Acad. Sci. USA 75(5): 2239–2243.
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Yamanaka, M., Nakamura, S., Inoue, A., Yasuda, T., Inoue, Y., Kawahara, H. (2010). Characterization of Cell-Sized Vesicles Induced from Human Lymphoid Cell Lines. In: Kamihira, M., Katakura, Y., Ito, A. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 16. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3892-0_9
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DOI: https://doi.org/10.1007/978-90-481-3892-0_9
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