Following our previous review on the progress in somatic embryogenesis in the Vitis genus until the beginning of this millennium (Martinelli and Gribaudo 2001), the present review reports the state-of the-art of this morphogenic process stressing the milestones, the most recent advances and the main points of interest. With this chapter, the authors aim to offer an experienced background to researchers who intend to study grape morphogenesis and to scientists who want to achieve successfully somatic embryogenesis in this fruitcrop.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Altamura MM, Cersosimo A, Majoli C, Crespan M (1992) Histological study of embryogenesis and organogenesis from anthers of Vitis rupestris du Lot cultured in vitro. Protoplasma 171:134-141
Altamura MM, Cersosimo A, Majoli C, Crespan M, Capitani F (1993) Formation of secondary embryoids obtained from anthers of Vitis rupestris Scheele cultured in vitro Giorn Bot Ital 127:297-299
Ammirato PV (1977) Hormonal control of somatic embryo development in cultured cells of caraway: interaction of abscisic acid, zeatin and gibberellic acid. Plant Physiol 59:579-586
Ammirato PV (1983) Embryogenesis. In: Evans DA, Sharp WR, Ammirato PV, Yamada Y (eds), Handbook of Plant Cell Cultures, Techniques for Propagation and Breeding. Mcmillan, New York
Ben Amar A, Cobanov P, Boonrod K, Krczal G, Bouzid S, Ghorbel AG, Reustle M (2007) Efficient procedure for grapevine embryogenic suspension establishment and plant regeneration: role of conditioned medium for cell proliferation. Plant Cell Rep 26:1439–1447
Bertsch C, Kieffer F, Maillot P, Farine S, Butterlin G, Merdinoglu D, Walter B (2005) Genetic chimerism of Vitis vinifera cv Chardonnay 96 is maintained through organogenesis but not somatic embryogenesis. BMC Plant Biol 5:20 (doi:101186/1471-2229-5-20)
Bertoldi D, Tassoni A, Martinelli L, Bagni N (2004) Polyamines and somatic embryogenesis in different Vitis vinifera L cultivars. Physiol Plant 120:657-666
Bornhoff B-A, Harst M (2000) Establishment of embryo suspension cultures of grapevines (Vitis L). Vitis 39:27-29
Bondaz J, Gambino G, Cuozzo D, Gribaudo I (2004) Risanamento di cloni di vite (Vitis vinifera L) da virus floematici mediante embriogenesi somatica. Petria 14:99-108
Bouamama B, Ben Salem-Fnayou A, Ben Jouira H, Ghorbel A, Mliki A (2007) Influence of the flower stage and culture medium on the induction of somatic embryogenesis from anther culture in Tunisian grapevine cultivars. J Int Sci Vigne Vin 41(4):185-192
Bouquet A (1989) Culture in vitro de la vigne Attention aux mauvaises surprises! Progr Agric Vitic 106:303-305
Bouquet A, Piganeau B, Lamaison M (1982) Influence du génotype sur la production de cals, d’embryoides et de plantes entières par culture d’anthères in vitro dans le genre Vitis Compt Bornhoff B-A, Hars Rend Acad Sci Paris 295:569-574
Cadavid-Labrada A, Medina C, Martinelli L, Arce-Johnson P (2008) Somatic embryogenesis and efficient regeneration of Vitis vinifera L cv Carménère plants. Vitis 47:73-74
Cao Z (1990) Grape anther culture. In: Zenghua Chen et al (ed), Handbook of Plant Cell Culture, Vol VI, Perennial Crops. McGraw-Hill, New York
Carimi F, Barizza E, Gardiman M, Lo Schiavo F (2005) Somatic embryogenesis from stigmas and styles of grapevine. In vitro Cell Dev Biol 41(3):249-252
Chugh A, Khurana P (2002) Gene expression during somatic embryogenesis – recent advances. Curr Sci 83:715-730
Chatelet P, Laucou V, Fernandez L, Sreekantan L, Lacombe T, Martinez-Zapater JM, Thomas M, Torregrosa RL (2007) Characterization of Vitis vinifera L somatic variants exhibiting abnormal flower development patterns. J Exp Bot 58:4107-4118
Colova-Tsolova V, Perl A, Krastanova S, Tsvetkov I, Atanassov A (2001) Genetically engineered grape for disease and stress tolerance. In: KA Roubelakis-Angelakis (ed) Molecular Biology and Biotechnology of the Grapevine. Kluwer Academic Publishers, The Netherlands
Colova-Tsolova VM, Bordallo PN, Phills BR, Bausher M (2007) Synchronized somatic embryo development in embryogenic suspensions of grapevine Muscadinia rotundifolia (Michx) Small. Vitis 46:15-18
Compton ME, Gray DJ (1996) Effects of sucrose and methylglyoxal bis-(guanylhydrazone) on controlling grape somatic embryogenesis. Vitis 35:1-6
Coutos-Thevenot P, Goebel-Tourand I, Mauro MC, Jouanneau JP, Boulay M, Deloire A, Guern J (1992a) Somatic embryogenesis from grapevine cells I-Improvement of embryo development by changes in culture conditions. Plant Cell Tiss Org Cult 29:125-133
Coutos-Thevenot P, Maes O, Jouenne T, Mauro MC, Boulay M, Deloire A, Guern J (1992b) Extracellular protein patterns of grapevine cell suspensions in embryogenic and nonembryogenic situations. Plant Sci 86:137-145
Cutanda MC, Bouquet A, Chatelet P, Lopez G, Botella O, Montero FJ, Torregrosa L (2008) Somatic embryogenesis and plant regeneration of Vitis vinifera cultivars ‘Macabeo’ and ‘Tempranillo’. Vitis 47:159-162
Das DK, Reddy MK, Upadhyaya KC, Sopory SK (2002) An efficient leaf-disc culture method for the regeneration via somatic embryogenesis and transformation of grape (Vitis vinifera L). Plant Cell Rep 20:999-1005
Das DK, Nirala NK, Reddy MK, Sopory SK, Upadhyaya KC (2006) Encapsulated somatic embryos of grape (Vitis vinifera L): an efficient way for storage and propagation of pathogenefree plant material. Vitis 45:179-184
Desperrier JM, Berger JL, Bessis R, Fournioux JC, Labroche C (2003) Création clonale dirigée par embryogenèse somatique. Bull OIV 871-872:751-765
de Vries SC (1998) Making embryos in plants. Trends Plant Sci 3:451-452
Dodeman VL, Ducreux G, Kreis M (1997) Zygotic embryogenesis versus somatic embryogenesis. J Exp Bot 48:1493-1509
Emershad RL, Ramming DW (1994) Somatic embryogenesis and plant development from immature zygotic embryos of seedless grapes (Vitis vinifera L). Plant Cell Rep 14:6-12
Esensee V, Stushnoff C, Forsline P (1990) Cryopreservation of dormant grape (Vitis spp) buds. HortSci 25:90
Faure O (1990) Embryons somatiques de Vitis rupestris et embryons zygotiques de Vitis sp: morphologie, histologie, histochimie et développement. Can J Bot 68:2305-2315
Faure O, Aarrouf J (1994) Metabolism of reserve products during development of somatic embryos and germination of zygotic embryos in grapevine. Plant Sci 96:167-178
Faure O, Nougarède A (1993) Nuclear DNA content of somatic and zygotic embryos of Vitis vinifera cv Grenache noir at the torpedo stage Flow cytometry and in situ DNA microspectrophotometry. Protoplasma 176:145-150
Faure O, Aarrouf J, Nougarède A (1996a) Ontogenesis, differentiation and precocious germination in anther-derived somatic embryos of grapevine (Vitis vinifera L): proembryogenesis. Ann Bot 78:23-8
Faure O, Aarrouf J, Nougarède A (1996b) Ontogenesis, differentiation and precocious germination in anther-derived somatic embryos of grapevine (Vitis vinifera L): embryonic organogenesis. Ann Bot 78:29-37
Faure O, Dewitte W, Nougarède A, VanOnckelen H (1998) Precociously germinating somatic embryos of Vitis vinifera have lower ABA and IAA levels than their germinating zygotic counterparts. Physiol Plant 102:591-595
Faure O, Mengoli M, Nougarède A, Bagni N (1991) Polyamine pattern and biosynthesis in zygotic and somatic embryo stages of Vitis vinifera. J Plant Physiol 138:545-549
Favre JM (1977) Premiers résultats concernant l’obtenition in vitro de néoformations aulinaires chez la Vigne. Ann Am Plantes 27:151-169
Finkelstein RR Crouch ML (1984) Precociously germinating rapeseed embryos retain characteristics of embryogeny. Planta 162:125-131
Franks T, He DG, Thomas M (1998) Regeneration of transgenic Vitis vinifera L Sultana plants: genotypic and phenotypic analysis. Mol Breed 4:321-333
Franks T, Botta RM, Thomas R (2002) Chimerism in grapevines: implications for cultivar identity, ancestry and genetic improvement. Theor Appl Genet 104:192-199
Gambino G, Bondaz J, Gribaudo I (2006) Detection and elimination of viruses in callus, somatic embryos and regenerated plantlets of grapevine. Eur J Plant Pathol 114:397-404
Gambino G, Ruffa P, Vallania R, Gribaudo I (2007) Somatic embryogenesis from whole flowers, anthers and ovaries of grapevine (Vitis spp). Plant Cell Tiss Org Cult 90(1):79-83
Gambino G, Di Matteo D, Gribaudo I (2009) Elimination of Grapevine fanleaf virus from three Vitis vinifera cultivars by somatic embryogenesis. Eur J Plant Pathol 123:57-60
Gianazza E, De Ponti P, Scienza A, Villa P, Martinelli L (1992) Monitoring by two-dimensional electrophoresis somatic embryogenesis in leaf and petiole explants from Vitis. Electrophoresis 13:203-209
Goebel-Tourand I, Mauro MC, Sossountazov L, Miginiac E, Deloire A (1993) Arrest of somatic embryo development in grapevine: histological characterization and the effect of ABA, BAP and zeatin in stimulating plantlet development. Plant Cell Tiss Org Cult 33:91-103
Goussard PG, Wiid J (1992) The elimination of fanleaf virus from grapevines using in vitro somatic embryogenesis combined with heat therapy. S Afr J Enol Vitic 13:81-83
Goussard PG, Wiid J, Kasdorf GGF (1991) The effect of in vitro somatic embryogenesis in eliminating fanleaf virus and leafroll associated viruses from grapevines. S Afr J Enol Vitic 12:77-81
Gray DJ (1987) Quiescence in monocotyledonous and dicotyledonous somatic embryos induced by dehydration. HortSci 22:810-814
Gray DJ (1989) Effects of dehydration and exogenous growth regulators on dormancy, quiescence and germination of grape somatic embryos. In vitro Cell Dev Biol 5:1173-1178
Gray DJ (1992) Somatic embryogenesis and plant regeneration from immature zygotic embryos of muscadine grapevine (Vitis rotundifolia) cultivars. Am J Bot 79:542-546
Gray DJ (1995) Somatic embryogenesis in grape. In: Jain S, Gupta P, Newton R (eds) Somatic embryogenesis in woody plants. Kluwer Acad Publ, NL
Gray DJ, Compton ME (1993) Grape somatic embryo dormancy and quiescence: potential of dehydrated synthetic seeds for germplasm conservation. In: Redenbaugh K (ed), Synseeds: applications of synthetic seeds to crop improvement. CRC Press, Boca Raton
Gray DJ, Meredith CP (1992) Grape. In: Hammerschlag FA, Litz RE (eds) Biotechnology of Perennial Fruit Crops. CAB Intern, Wallingford, UK
Gray DJ, Mortensen JA (1987) Initiation and maintenance of long term somatic embryogenesis from anthers and ovaries of Vitis longii ‘Microsperma’. Plant Cell Tiss Org Cult 9:73-80
Gray DJ, Jayasankar S, Litz RE (2002) Regeneration system for grape and uses thereof. USA Patent No 6,455,312 B1, Sept 24, 2002
Gresshoff PM, Doy CH (1974) Derivation of a haploid cell line from Vitis vinifera and the importance of the stage of meiotic development of anthers for haploid culture of this and other genera. Z Pflanzenphysiol 73:123-141
Gribaudo I, Gambino G, Vallania R (2004) Somatic embryogenesis from grapevine anthers: the optimal developmental stage for collecting explants. Am J Enol Vitic 55:427-430
Gribaudo I, Gambino G, Cuozzo D, Mannini F (2006) Attempts to eliminate Grapevine rupestris stem pitting-associated virus from grapevine clones. J Plant Pathol 88:293-298
Gribaudo I, Torello Marinoni D, Gambino G, Mannini F (2009) Assessment of genetic fidelity in somaclones of two Vitis vinifera cultivars. Acta Hortic (in press)
Harst M (1995) Development of a regeneration protocol for high frequency somatic embryogenesis from explants of grapevines (Vitis spp). Vitis 34:27-29
Hirabayashi T, Kozaki I, Akihama T (1976) In vivo differentiation of shoots from anther callus in Vitis grape research. HortSci 11:511-512
Hocquigny S, Pelsy F, Dumas V, Kindt S, Heloir M-C, Merdinoglu D (2004) Diversification within grapevine cultivars goes through chimeric states. Genome 47:579-589
Jayasankar S, Gray DJ, Litz RE (1999) High-efficiency somatic embryogenesis and plant regeneration from suspension cultures of grapevine. Plant Cell Rep 18:533-537
Jayasankar S, Li Z, Gray DJ (2000) In-vitro selection of Vitis vinifera ‘Chardonnay’ with Elsinoe ampelina culture filtrate is accompanied by fungal resistance and enhanced secretion of chitinase. Planta 211:200-208
Jayasankar S, Bondada BR, Li Z, Gray DJ (2002) A unique morphotype of grapevine somatic embryos exhibits accelerated germination and early plant development. Plant Cell Rep 20:907- 911
Jayasankar S, Bondada B, Li Z, Gray DJ (2003) Comparative anatomy and morphology of Vitis vinifera (Vitaceae) somatic embryos form solid- and liquid-culture-derived proembryogenic masses. Am J Bot 90:973-979
Jayasankar S, Van Aman M, Li Z, Gray DJ (2001) Direct seeding of grapevine somatic embryos and regeneration of plants. In vitro Cell Dev Biol-Plant 37:476-479
Jayasankar S, Van Aman M, Codts J, Li Z, Gray DJ (2005) Low temperature storage of suspension culture-derived grapevine somatic embryos and regeneration of plants. In vitro Cell Dev Biol-Plant 41:752-756
Jiménez VM, Bangerth F (2000) Relationship between endogenous hormone levels of grapevine callus cultures and their morphogenetic behaviour. Vitis 39:151-157
Jittayasothorn Y, Lu J, Xu X, Thipyapong P, Boonkerd N (2007) A simple and highly efficient protocol for somatic embryogenesis and plant regeneration from proembryonic mass suspension culture in ‘Autumn Royal Seedless’. Vitis 46:45-46
Kikkert JR, Thomas MR, Reisch BI (2001) Grapevine genetic engineering. In: Roubelakis- Angelakis KA (ed) Molecular Biology and Biotechnology of the Grapevine. Kluwer Acad Publ, NL
Kikkert JR, Striem MJ, Vidal JR, Wallace P, Barnard, G, Reisch B (2005a) Long-term study of somatic embryogenesis from anthers and ovaries of 12 grapevine (Vitis sp) genotypes In vitro. Cell Dev Biol-Plant 41:232-239
Kikkert JR, Vidal JR, Reisch BI (2005b) Stable transformation of plant cells by particle bombardment/biolistics. Methods Mol Biol 286:61-78
Krul WR, Narragansett RI (1985) In vitro propagation of vine. USA Patent No 4,532,733, Aug 6, 1985
Krul, WR, Worley JF (1977) Formation of adventitious embryos in callus cultures of ‘Seyval’, a French hybrid grape. J Am Soc Hort Sci 102:360-363
Krul ER, Mowbray GH (1984) Grapes. In: Sharp WR, Evans D, Ammirato PV, Yamada Y (eds), Handbook of Plant Cell Cultures. Vol II, Crop Species, Mcmillan, New York
Kuksova VB, Piven NM, Gleba YY (1997) Somaclonal variation and in vitro induced mutagenesis in grapevine. Plant Cell Tiss Org Cult 49:17-27
Leal F, Pais MS, Ortiz JM, Guedes-Pinto H, Pinto-Carnide O (2008) Molecular analysis of Vitis vinifera L plants obtained by somatic embryogenesis. Acta Hortic (in press)
Lebrun L, Branchard M (1986) Embryogenèse ‘indefinie’ chez Vitis sp cultivé in vitro. Proc 3° Symp Intern sur Physiologie de la Vigne, 24-27 June 1986, Bordeaux, France
Lápez-Pérez J, Carreño J, MartÃnez-Cutillas A, Dabauza M (2005) High embryogenic ability and plant regeneration of table grapevine cultivars (Vitis vinifera L) induced by activated charcoal. Vitis 44:79-85
Maës O, Coutos-Thévenot P, Jouenne T, Boulay M, Guern J (1997) Influence of extracellular proteins, proteases and protease inhibitors on grapevine somatic embryogenesis. Plant Cell Tiss Org Cult 50:97-105
Maillot P, Kieffer F, Walter B (2006) Somatic embryogenesis from stem nodal sections of grapevine. Vitis 45:185-189
Maitz M, Trampler F, Gröschl M, da Câmara Machado, A, Laimer da Câmara Machado M (2000) Use of an ultrasound cell retention system for the size fractionation of somatic embryos of woody species. Plant Cell Rep 19:1057–1063
Marchenko AO (1991) Induction of embryogenesis in primary calluses from grape stems and leaves. Fiziologiya Rastenii 38:580-591
Margosan DA, Emershad RL, Ramming DW (1994) Origin of somatic embryos from embryo rescue cultures of seedless grapes (Vitis vinifera L). Proc Intern Symp on Table Grape Production, 28-29 June 1994, Anaheim, California
Marsoni M, Bracale M, Espen L, Prinsi B, Negri AS, Vannini C (2008) Proteomic analysis of somatic embryogenesis in Vitis vinifera. Plant Cell Rep 27:347–356
Martinelli L, Mandolino G (1994) Genetic transformation and regeneration of transgenic plants in grapevine (Vitis rupestris S). Theor Appl Genet 88:621-628
Martinelli L, Mandolino G (2001) Genetic transformation in Vitis. In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry. Springer-Verlag, Berlin
Martinelli L, Gribaudo I (2001) Somatic embryogenesis in grapevine (Vitis spp). In: Roubelakis- Angelakis KA (ed) Molecular Biology and Biotechnology of the Grapevine. Kluwer Acad Publ, NL
Martinelli L, Bragagna P, Poletti V, Scienza A (1993a) Somatic embryogenesis from leaf- and petiole-derived callus of Vitis rupestris. Plant Cell Rep 12:207-210
Martinelli L, Scienza A, Villa P, De Ponti, P, Gianazza E (1993b) Enzyme markers for somatic embryogenesis in Vitis. J Plant Physiol 141:476-481
Martinelli L, Buzkan N, Minafra A, Saldarelli P, Costa D, Poletti V, Festi S, Perl A, Martelli GP (2000) Genetic transformation of tobacco and grapevines for resistance to viruses related to the rugose wood disease complex. Acta Hort 528:321-327
Martinelli L, Candioli E, Costa D, Poletti V, Rascio N (2001a) Morphogenic competence of secondary somatic embryos with a long culture history. Plant Cell Rep 20:279-284
Martinelli L, Gribaudo I, Bertoldi D, Candioli E, Poletti V (2001b) High efficiency somatic embryogenesis and plant germination in grapevine cultivars Chardonnay and Brachetto a grappolo lungo. Vitis 40:111-115
Martinelli L, Candioli E, Costa D, Minafra A (2002) Stable insertion and expression of the movement protein gene of Grapevine Virus A (GVA) in grape lines (Vitis rupestris S). Vitis 41:189-193
Martinelli L, Gribaudo I, Semenzato M, Poletti V, Gambino G (2003) Ovary as valuable explant for somatic embryogenesis induction in grapes (Vitis spp). Acta Hort 603:499-504
Martinelli L, Zambanini J, Grando S (2004) Genotype assessment of grape regenerants from floral explants. Vitis 43:119-122
Matsuta N, Hirabayashi T (1989) Embryogenic cell lines from somatic embryos of grape (Vitis vinifera L). Plant Cell Rep 7:684-687
Mauro MC, Nef C, Fallot J (1986) Stimulation of somatic embryogenesis and plant regeneration from anther culture of Vitis vinifera cv Cabernet Sauvignon. Plant Cell Rep 5:377-380
Mordhorst AP, Toonen MAJ, de Vries S (1997) Plant embryogenesis. Plant Sci 16:535-576
Morgana C, Di Lorenzo R, Carimi F (2004) Somatic embryogenesis of Vitis vinifera L (cv Sugraone) from stigma and style culture. Vitis 43:169-173
Motoike SY, Skirvin RM, Norton MA, Ottenbacher AG (2001) Somatic embryogenesis and long term maintenance of embryogenic lines from fox grapes. Plant Cell Tiss Org Cult 66:121-131
Mozsar J, Sule S (1994) A rapid method for somatic embryogenesis and plant regeneration from cultured anthers of Vitis riparia. Vitis 33:245-246
Mozsar J, Viczian O (1996) Genotype effect on somatic embryogenesis and plant regeneration of Vitis spp. Vitis 35:155-157
Mullins MG (1990) Applications of tissue culture to the genetic improvement of grapevine. Proc 5th Intern Symp on Grape Breeding. Vitis (sp issue):399-407
Mullins MG, Rajasekaran K (1980) Plantlets from cultured anthers of Vitis species and hybrids. Proc 3dt Int Symp on Grape Breeding, Davis, CA :111-119
Mullins MG, Srinivasan C (1976) Somatic embryos and plantlets from an ancient clone of the grapevine (cv Cabernet Sauvignon) by apomixis in vitro. J Exp Bot 27:1022-1030
Mullins MG, Tang FCA, Facciotti D (1990) Agrobacterium-mediated genetic transformation of grapevines: transgenic plants of Vitis rupestris Scheele and buds of Vitis vinifera L. Bio/Technology 8:1041-1045
Nakajima I, Matsuda N (2003) Somatic embryogenesis from filaments of Vitis vinifera L, Vitis labruscana Bailey. Vitis 42:53-54
Nakajima I, Kobayashi S, Nakamura Y (2000) Embryogenic callus induction and plant regeneration from unfertilized ovule of ‘Kyoho’ grape. J Japan Soc Hortic Sci 69:186-188
Nakano M, Sakakibara T, Watanabe Y, Mii M (1997) Establishment of embryogenesis cultures in several cultivars of Vitis vinifera and V. labruscana. Vitis 36:141-145
Nakano M, Watanabe Y, Hoshino Y (2000) Histological examination of callogenesis and adventitious embryogenesis in immature ovary culture of grapevine (Vitis vinifera L). J Hortic Sci Biotechnol 75:154-160
Newton DJ, Goussard PG (1990) The ontogeny of somatic embryos from in vitro cultured grapevine anthers. S Afr J Enol Vitic 11:70-75
Nookaraju A, Barreto MS, Agrawal DC (2008) Cellular polyamines influence maturation and germination of somatic embryos from pro-embryonal masses of two grapevine cultivars. Vitis 47:31-34
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473-497
Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163:85-87
Pearce D, Pharis RP, Rajasekaran K, Mullins MG (1987) Effects of chilling and ABA on (3H)gibberellin A4 metabolism in somatic embryos of grape (Vitis vinifera L x V rupestris Scheele). Plant Physiol 80:381-385
Perl A, Saad S, Sahar N, Holland D (1995) Establishment of long-term embryogenic cultures of seedless Vitis vinifera cultivars – a synergistic effect of auxins and the role of abscisic acid. Plant Sci 104:193-200
Perl A, Lotan O, Abu-Abied M, Holland D (1996) Establishment of an Agrobacterium-mediated transformation system for grape (Vitis vinifera L): The role of antioxidants during grape–Agrobacterium interactions. Nature Biotech14:624-628
Perl A, Eshdat Y (2007) Grape. In: Pua EC, Davey MR (eds) Transgenic Crops V Biotechnology in Agriculture and Forestry. Springer-Verlag, Berlin, pp 189-208
Perrin M, Martin D, Joly D, Demangeat G, This P, Masson JE (2001) Medium-dependent response of grapevine somatic embryogenic cells. Plant Sci 161:107-116
Perrin M, Gertz C, Masson JE (2004) High efficiency initiation of regenerable embryogenic callus from anther filaments of 19-grapevine genotypes grown worldwide. Plant Sci 167:1343-49
Pinto-Sintra AL, (2007) Establishment of embryogenic cultures and plant regeneration in the Portuguese cultivar Touriga Nacional of Vitis vinifera L. Plant Cell Tiss Org Cult 88:253-265
Popescu CF, Falk A, Glimelius K (2002) Application of AFLPs to characterize somaclonal variation in anther-derived grapevines. Vitis 41:177-182
Quiroz-Figueroa RF, Rojas-Herrera R, Galaz-Avalos RM, Loyola-Vargas VM (2006) Embryo production through somatic embryogenesis can be used to study cell differentiation in plants. Plant Cell Tiss Org Cult 86:285-301
Rajasekaran K, Mullins MG (1979) Embryo and plantlets from cultured anthers of hybrid grapevines. J Exp Bot 30:399-407
Rajasekaran K, Mullins MG (1983) The origin of embryos and plantlets from cultured anthers of hybrid grapevines. Am J Enol Vitic 34:108-113
Rajasekaran K, Vine J, Mullins MG (1982) Dormancy in somatic embryos and seeds of Vitis: changes in endogenous abscisic acid during embryogeny and germination. Planta 154:139-144
Redenbaugh K, Paasch BD, Nichol JW, Kossler ME, Viss PR, Walker KA (1986) Somatic seeds: encapsulation of asexual plant embryos. Bio/Technol 4:797-801
Redon C, Albert P, Lacorre-Arescaldino I, Mauro MC, Bierne J (1996) Transition in histone H1 from globular to torpedo stage in grapevine somatic embryos. Proc Intern Congress French Soc Developm Biol (SFBD), Maternal and zygotic genes during embryonic development. May 29-31, 1996, Caen, F, A57 P, 35S
Redon C, Albert P, Lacorre-Arescaldino I, Mauro MC, Bierne J (1999) Electrophoretic heterogeneity of grapevine histones. Am J Enol Vitic 50:13-18
Reinert J (1958) Untersuchungen uber die Morphogenese an Gewebekulturen. Ber Dtch Ges 71:15
Reustle G, Harst M, Alleweldt G (1995) Plant regeneration of grapevine (Vitis sp) protoplasts isolated from embryogenic tissues. Plant Cell Rep 15:238-241
Robacker C (1993) Somatic embryogenesis and plant regeneration from muscadine grape leaf explants. HortSci 28:53-55
Salunkhe CK, Rao PS, Mhatre M (1997) Induction of somatic embryogenesis and plantlets in tendrils of Vitis vinifera L. Plant Cell Rep 17:65-7
Salunkhe CK, Rao PS, Mhatre M (1999) Plantlet regeneration via somatic embryogenesis in anther callus of Vitis latifolia L. Plant Cell Rep 18:670-673
Scorza R, Cordts JM, Ramming DW, Emershad RL (1995) Transformation of grape (Vitis vinifera L) zygotic-derived somatic embryos and regeneration of transgenic plants. Plant Cell Rep 14:589-592
Scorza R, Cordts JM, Gray DJ, Gonsalves D, Emershad RL, Ramming DW (1996) Producing transgenic Thompson Seedless grape (Vitis vinifera L). J Amer Soc Hort Sci 12:616-619
Sefc KM, Ruckenbauer P, Regner F (1997) Embryogenesis in microspore culture of Vitis subspecies. Vitis 36:15-20
Skene KGM, Goodwins DR, Barlass M (1988) Ploidy stability in grapevines following long term storage in vitro. Vitis 27:41-44
Soulie O, Roustan J-P, Fallot J (1993) Early in vitro selection of eutypine-tolerant plantlets Application to screening of Vitis vinifera cv Ugni blanc somaclones. Vitis 32:243-244
Srinivasan C, Mullins MG (1980) High-frequency somatic embryo production from unfertilized ovules of grapes. Sci Hortic 13:245-252
Stamp JA, Meredith CP (1988a) Somatic embryogenesis from leaves and anthers of grapevine. Sci Hortic 35:235-250
Stamp JA, Meredith CP (1988b) Proliferative somatic embryogenesis from zygotic embryos of grapevine. J Amer Soc Hort Sci 113:941-945
Steward FC, Mapes MO, Mears M (1958) Growth and organized development of cultures cells. II Organization in cultures grown from freely suspended cells. Am J Bot 45:705-708
Takeno K, Koshioka M, Pharis RP, Rajasekaran K, Mullins MG (1983) Endogenous gibberellinlike substances in somatic embryos of grape (Vitis vinifera x Vitis rupestris) in relation to embryogenesis and the chilling requirement for subsequent development of mature embryos. Plant Physiol 73:803-808
Taylor RL (1967) The foliar embryos of Malaxis paludosa. Can J Bot 45:1553-1556
This P, Jung A, Boccacci P, Borrego J, Botta R, Costantini L, Crespan M, Dangl GS, Eisenheld C, Ferreira-Monteiro F, Grando MS, Ibanez J, Lacombe T, Laucou V, Magalhaes R, Meredith CP, Milani N, Peterlunger E, Regner F, Zulini, L, Maul E (2004) Development of a standard set of microsatellite reference alleles for identification of grape cultivars. Theor Appl Gen 109:1448-1458
Torregrosa L (1995) Biotechnologie de la vigne: les techniques de régénération in vitro (synthèse). Progr Agric Vitic 112:479-489
Torregrosa L (1998) A simple and efficient method to obtain stable embryogenic cultures from anthers of Vitis vinifera L. Vitis 37:91-92
Torregrosa L, Bouquet, A, Goussard PG (2001) In vitro culture and propagation of grapevine. In: Roubelakis-Angelakis KA (ed) Molecular Biology and Biotechnology of the Grapevine. Kluwer Acad Publ, NL
Torres-Viñals M, Sabaté-Casaseca S, Aktouche N, Grenan S, Lopez G, Porta-Falguera M, Torregrosa L (2004) Large-scale production of somatic embryos as a source of hypocotyl explants for Vitis vinifera micrografting. Vitis 43:163-168
Tsolova V, Atanassov A (1996) Plant regeneration of Vitis sp via repetitive embryogenesis. Biotechnol & Biotechnol Eq 10/1996/1:32-34
Vilaplana M, Mullins MG (1989) Regeneration of grapevine (Vitis spp) in vitro: formation of adventitious buds on hypocotyls and cotyledons of somatic embryos. J Plant Physiol 134:413-419
Yarbrough YA (1932) Anatomical and developmental studies of the foliar embryos of Bryphyllum calycinum. Am J Bot 19:443-453
Wang Q, Mawassi M, Sahar N, Li P, Colova-Tsolova V, Sela I, Gafny R, Tanne, E, Perl A (2004) Cryopreservation of grapevine (Vitis vinifera L) embryogenic cell suspensions by encapsulation-vitrification. Plant Cell Tiss Org Cult 77:267-275
Xu X, Lu J (2009) Somatic embryogenesis from seed integuments of seedless grape cultivars. Acta Hortic (in press)
Xu X, Lu J, Dalling D, Jattayasothorn Y, Grosser JW (2007) Isolation and culture of grape protoplasts from embryogenic suspension cultures and leaves of Vitis vinifera and Vitis rotundifolia. Acta Hortic 738:787-790
Yang XM, Cao ZY, An LZ, Wang YM, Fang XW (2006) In vitro tetraploid induction via colchicine treatment from diploid somatic embryos in grapevine (Vitis vinifera L). Euphytica 152:217-224
Zhu YM, Hoshino Y, Nakano M, Takahashi E, Mii M (1997) Highly efficient system of plant regeneration from protoplasts of grapevine (Vitis vinifera L) through somatic embryogenesis by using embryogenic callus culture and activated charcoal. Plant Sci 123:151-157
Zlenko VA, Kotikov IV, Troshin LP (2002) Efficient GA3-assisted plant regeneration from cell suspensions of three grape genotypes via somatic embryogenesis. Plant Cell Tiss Org Cult 70:295-299
Zlenko VA, Kotikov IV, Troshin LP (2005) Effects of IAA and BA on development of globular, heart- and torpedo-stage embryos from cell suspensions of three grape genotypes. Sci Hortic 104:237-247
Zou CJ, Li PF (1981) Induction of pollen plants of grape (Vitis vinifera L). Acta Bot Sinica 23:79-81
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Springer Science+Business Media B.V.
About this chapter
Cite this chapter
Martinelli, L., Gribaudo, I. (2009). Strategies for Effective Somatic Embryogenesis in Grapevine: An appraisal. In: Roubelakis-Angelakis, K.A. (eds) Grapevine Molecular Physiology & Biotechnology. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-2305-6_17
Download citation
DOI: https://doi.org/10.1007/978-90-481-2305-6_17
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-2304-9
Online ISBN: 978-90-481-2305-6
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)