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Multipotent Stromal Cells (hMSCs)

  • Chapter
Human Adult Stem Cells

Part of the book series: Human Cell Culture ((HUCC,volume 7))

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Abstract

The existence of the non-hematopoietic stem/progenitor cells from bone marrow known as mesenchymal stem cells, marrow stromal cells, or multipotent mesenchymal stromal cells (MSCs), was first suggested over a hundred years ago. Definitive evidence that bone marrow contains cells that can differentiate into fibroblasts as well as other mesenchymal cells has been available since the mid-1970s. Over the last three decades, a great deal of research has been conducted on MSCs in laboratories worldwide. It has been found that these cells are easily isolated from small volumes of bone marrow, can be expanded to large numbers in a relatively short period of time with basic tissue culture techniques and can undergo differentiation into several different tissue types. In addition, it has been shown that MSCs are a part of the body’s natural repair mechanism and, thus, there has been great interest in using MSCs for treatment of various diseases and injuries, such as diabetes, chronic heart failure, Parkinson’s disease, Alzheimer’s disease, and spinal cord injury, to name just a few. Although the knowledge of these cells has grown exponentially and interest in MSCs has increased proportionally, each lab has developed their own protocols and methods of isolation, culture and characterization, thus, making it difficult to compare the results from experiments with MSCs from different labs. In spite of the ease with which these cells are isolated and cultured, there are some important criteria for the culture of these cells which must be observed in order to produce MSCs which have the capability to expand, multidifferentiate, form colonies and also to perform well when used for in vitro and in~vivo studies and in clinical therapies. This chapter will cover human MSC isolation, expansion, characterization and potential therapeutic uses.

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Author information

Authors and Affiliations

  1. Center for Gene Therapy, Tulane University Health Sciences, New Orleans, LA, 70112, USA

    Margaret Wolfe & Alan Tucker

  2. Institute for Regenerative Medicine at Scott and White, Texas A&M Health Sciences Center, Temple, TX, 76502, USA

    Roxanne L. Reger & Darwin J. Prockop

Authors
  1. Margaret Wolfe
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  2. Alan Tucker
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  3. Roxanne L. Reger
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  4. Darwin J. Prockop
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Corresponding author

Correspondence to Margaret Wolfe .

Editor information

Editors and Affiliations

  1. University College London Inst. Urology & Nephrology, 67 Riding House Street, London, United Kingdom W1P 7PN

    John R. Masters

  2. Dept. Bioengineering, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093-0412, USA

    Bernhard Ø. Palsson

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Wolfe, M., Tucker, A., Reger, R.L., Prockop, D.J. (2009). Multipotent Stromal Cells (hMSCs). In: Masters, J.R., Palsson, B.Ø. (eds) Human Adult Stem Cells. Human Cell Culture, vol 7. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-2269-1_2

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