Abstract
RNA interference (RNAi) is a recently discovered technique for the directed inhibition of gene expression at the level of the mRNA. Its specificity and potency make it a promising method for manipulating cellular phenotypes in eukaryotic cell lines and primary cells. RNAi is already being explored for therapeutic and bioprocessing applications, and it is expected that this will only expand as understanding of the mechanism increases. In this chapter, the mechanism of RNAi is described, followed by a description of some of the technical challenges that remain to realize the full benefits of RNAi. The progress to date on RNAi-based applications will also be detailed.
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Financial support for this work was provided in part by Michigan State University, the National Science Foundation (#0425821), and the National Institutes of Health (#CA126136, #GM079688, #RR024439).
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Gredell, J.A., Kini, H.K., Walton, S.P. (2009). Engineering Cell Function by RNA Interference. In: Al-Rubeai, M. (eds) Cell Line Development. Cell Engineering, vol 6. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-2245-5_9
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