Abstract
Fine-needle biopsy (FNB) is a convenient and reliable first-line diagnostic procedure for the investigation of superficial and deep seated lymphadenopathies. Its application can vary according to the clinical context, the age of the patient, and the anatomic site of the lesion [1–3]. Morphological assessment of the cellular sample serves as the basis for identifying several inflammatory conditions of the lymph nodes, as well as most highgrade lymphoid malignancies and non-lymphoid metastatic tumors, but it has significant limitations in the study of low-grade lymphomas as it does not allow their differentiation from several reactive conditions [4–7]. However, this limitation can be overcome by the use of ancillary laboratory techniques in FNB samples, as these often reveal important immunophenotypic and biomolecular information that assists in obtaining a definitive diagnosis. Finally, a tissue incisional biopsy, using cutting needles, can be performed in parallel with FNB sampling of the lymph node. This approach may add relevant morphological data to the cytological evaluation as well as provide a significant amount of cellular sample for ancillary techniques.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Lioe TFEH, Allen DC, Spence RA (1999) The role of fine needle aspiration cytology (FNAC) in the investigation of superficial lymphadenopathy; uses and limitations of the technique. Cytopathology 10:291–297.
Kocjan G (1997) The role of FNAC in diagnosis of lymph node enlargements. Cytopathology 8 (suppl):2–3.
Liu ES, Bernstein JM, Sculerati N et al (2001) Fine needle aspiration biopsy of pediatric head and neck masses. Int J Pediatr Otorhinolaryngol 30:135–140.
Das DK (1999) Value and limitations of fine-needle aspiration cytology in diagnosis and classification of lymphomas: a review. Diagn Cytopathol 21:240–249.
Hehn ST, Grogan TM, Miller TP (2004) Utility of fine-needle aspiration as a diagnostic technique in lymphoma. J Clin Oncol 22:3046–3052.
Dong HY, Harris NL, Preffer FI et al (2001) Fine-needle aspiration biopsy in the diagnosis and classification of primary and recurrent lymphoma: a retrospective analysis of the utility of cytomorphology and flow cytometry. Mod Pathol 14:472–481.
Daskalopoulou D, Harhalakis N, Maouni N et al (1995) Fine needle aspiration cytology of non-Hodgkin’s lymphomas. A morphologic and immunophenotypic study. Acta Cytol 39:180–186.
Katz R (1997) Pitfalls in the diagnosis of fine-needle aspiration of lymph nodes. Monogr Pathol 39:118–33.
Liu K, Mann KP, Vitellas KM et al (1999) Fine-needle aspiration with flow cytometric immunophenotyping for primary diagnosis of intra-abdominal lymphomas. Diagn Cytopathol 21:98–104.
Henrique RM, Sousa ME, Godinho MI et al (1999) Immunophenotyping by flow cytometry of fine needle aspirates in the diagnosis of lymphoproliferative disorders: a retrospective study. J Clin Lab Anal 13:224–228.
Meda BA, Buss DH, Woodruff RD et al (2000) Diagnosis and subclassification of primary and recurrent lymphoma. The usefulness and limitations of combined fine-needle aspiration cytomorphology and flow cytometry. Am J Clin Pathol 113:688–699.
Young NA, Al-Saleem T (1999) Diagnosis of lymphoma by fine-needle aspiration cytology using the revised European-American classification of lymphoid neoplasms. Cancer 87:325–345.
Mourad WA, Tulbah A, Shoukri M et al (2003) Primary diagnosis and REAL/WHO classification of non-Hodgkin’s lymphoma by fine-needle aspiration: cytomorphologic and immunophenotypic approach. Diagn Cytopathol 28: 191–195.
Landgren O, Porwit MacDonald A, Tani E et al (2004) A prospective comparison of fine-needle aspiration cytology and histopathology in the diagnosis and classification of lymphomas. Hematol J 5:69–76.
Mayall F, Johnson S (2007) Immunoflow cytometry compared with PCR for the identification of clonality in FNAs of T-cell-rich B-cell lymphomas. Cytopathology 18:117–119.
Caraway NP, Gu J, Lin P, Romaguera J et al (2005) The utility of interphase fluorescence in situ hybridization for the detection of the translocation t(11;14)(q13;q32) in the diagnosis of mantle cell lymphoma on fine-needle aspiration specimens. Cancer 105:110–118.
Safley AM, Buckley PJ, Creager AJ et al (2004) The value of fluorescence in situ hybridization and polymerase chain reaction in the diagnosis of B-cell non-Hodgkin lymphoma by fine-needle aspiration. Arch Pathol Lab Med 128:1395–1403.
Jeffers MDMJ, Farquharson MA, Stewart CJ et al (1997) Analysis of clonality in cytologic material using the polymerase chain reaction (PCR). Cytopathology 8:114–121.
Alkan SLC, Sarago C, Sidawy MK et al (1995) Polymerase chain reaction detection of immunoglobulin gene rearrangement and bcl-2 translocation in archival glass slides of cytologic material. Diagn Mol Pathol 4:25–31.
Aiello ADD, Giardini R, Alasio L et al (1997) PCR analysis of IgH and BCL2 gene rearrangement in the diagnosis of follicular lymphoma in lymph node fine-needle aspiration. A critical appraisal. Diagn Mol Pathol 6:154–160.
Jiang F, Katz RL (2002) Use of interphase fluorescence in situ hybridization as a powerful diagnostic tool in cytology. Diagn Mol Pathol 11:47–57.
Haralambieva E, Banham AH, Bastard C et al (2003) Detection by the fluorescence in situ hybridization technique of MYC translocations in paraffin-embedded lymphoma biopsy samples. Br J Haematol 121:49–56.
Gong JZ, Snyder MJ, Lagoo AS et al (2004) Diagnostic impact of core-needle biopsy on fine-needle aspiration of non-Hodgkin lymphoma. Diagn Cytopathol 31:23–30.
Swerdlow SH, Campo E, Harris NL et al (eds) (2008) World Health Organization Classification of Tumours of Haematopoietic and Lymphoid Tissues. IARC Press, Lyon.
Chen L, Kuriakose P, Hawley RC et al (2005). Hematologic malignancies with primary retroperitoneal presentation: clinicopathologic study of 32 cases. Arch Pathol Lab Med 129:655–660.
Flanders E, Kornstein MJ, Wakely PE Jr et al (1993) Lymphoglandular bodies in fine-needle aspiration cytology smears. Am J Clin Pathol. 5:566–569.
Khurana KK, Stanley MW, Powers CN, Pitman MB (1998) Aspiration cytology of malignant neoplasms associated with granulomas and granuloma-like features: diagnostic dilemmas. Cancer 84:84–91.
Pilotti S, Di Palma S, Alasio L et al (1993) Diagnostic assessment of enlarged superficial lymph nodes by fine needle aspiration. Acta Cytol 37:853–866.
Sudilovsky D, Cha I (1998) Fine needle aspiration cytology of dermatopathic lymphadenitis. Acta Cytol 42:1341–1346.
Das DK, Gulati A, Bhatt NC (2001) Sinus histiocytosis with massive lymphadenopathy (Rosai-Dorfman disease): report of two cases with fine-needle aspiration cytology. Diagn Cytopathol 24:42–45.
Kardos TF, Kornstein MJ, Frable WJ (1988) Cytology and immunocytology of infectious mononucleosis in fine needle aspirates of lymph nodes. Acta Cytol 32:722–726.
Stanley MW, Steeper TA, Horwitz CA et al (1990). Fine-needle aspiration of lymph nodes in patients with acute infectious mononucleosis. Diagn Cytopathol 6:323–329.
Reid AJ, Miller RF, Kocjan GI (1998) Diagnostic utility of fine needle aspiration cytology in HIV-infected patients with lymphadenopathy. Cytopathology 9:230–239.
Lowe SM, Kocjan GI, Edwards SG, Miller RF (2008) Diagnostic yield of fine-needle aspiration cytology in HIVinfected patients with lymphadenopathy in the era of highly active antiretroviral therapy. Int J STD AIDS 19:553–556.
Kucukardali Y, Solmazgul E, Kunter E et al (2007) Kikuchi-Fujimoto Disease: analysis of 244 cases. Clin Rheumatol 26:50–54.
Tong TR, Chan OW, Lee KC (2001) Diagnosing Kikuchi disease on fine needle aspiration biopsy: a retrospective study of 44 cases diagnosed by cytology and 8 by histopathology. Acta Cytol 45:953–957.
Tsang WY, Chan JK (1994) Fine-needle aspiration cytologic diagnosis of Kikuchi’s lymphadenitis. A report of 27 cases. Am J Clin Pathol 102:454–458.
Hsueh EJ, Ko WS, Hwang WS, Yam LT (1993) Fine-needle aspiration of histiocytic necrotizing lymphadenitis (Kikuchi’s disease). Diagn Cytopathol 19:448–452.
Pileri SA, Ascani S, Leoncini L et al (2002) Hodgkin’s lymphoma: the pathologist’s viewpoint. J Clin Pathol 55:162–176.
Zhang JR, Raza AS, Greaves TS, Cobb CJ (2006) Fine-needle aspiration diagnosis of Hodgkin lymphoma using current WHO classification-re-evaluation of cases from 1999–2004 with new proposals. Diagn Cytopathol 34: 397–402.
Gatter KC, Warnke (2001) Diffuse large B-cell lymphoma. In: Jaffe ES, Harris NL, Stein H, Vardiman JW (eds) Pathology and Genetics of Tumours of Hematopoietic and Lymphoid Tissues. World Health Organization Classification of Tumours. IARC Press, Lyon, pp 171–176.
de Leval L, Harris NL (2003) Variability in immunophenotype in diffuse large B-cell lymphoma and its clinical relevance. Histopathology 43:509–528.
Rosenwald A, Wright G, Chan WC et al (2002) The use of molecular profiling to predict survival after chemotherapy for diffuse large-B-cell lymphoma. N Engl J Med 346:1937–1947.
Boleti E, Johnson PW (2007) Primary mediastinal B-cell lymphoma. Hematol Oncol 25:157–163.
Silverman JF, Raab SS, Park HK (1993) Fine-needle aspiration cytology of primary large-cell lymphoma of the mediastinum: cytomorphologic findings with potential pitfalls in diagnosis. Diagn Cytopathol 9:209–214.
Wright DH (1999) What is Burkitt’s lymphoma and when is it endemic? Blood 93:758.
Knowles DM (1996) Etiology and pathogenesis of AIDSrelated non-Hodgkin’s lymphoma. Hematol Oncol Clin North Am. 10:1081–1109.
Das DK, Gupta SK, Pathak IC et al (1987) Burkitt-type lymphoma. Diagnosis by fine needle aspiration cytology. Acta Cytol 31:1–7.
Powers CN, Wakely PE Jr, Silverman JF et al (1990) Fine needle aspiration biopsy of extramedullary plasma cell tumors. Mod Pathol 3:648–653.
Pai RR, Raghuveer CV (1996) Extramedullary plasmacytoma diagnosed by fine needle aspiration cytology. A report of four cases. Acta Cytol 40:963–966.
Das DK, Francis IM, Sharma PN et al (2009) Hodgkin’s lymphoma: Diagnostic difficulties in fine-needle aspiration cytology. Diagn Cytopathol 16. [Epub ahead of print]
Mourad WA, al Nazer M, Tulbah A (2003) Cytomorphologic differentiation of Hodgkin’s lymphoma and Ki-1+ anaplastic large cell lymphoma in fine needle aspirates. Acta Cytol 47:744–748.
Harris NL (1999) Hodgkin’s disease: classification and differential diagnosis. Mod Pathol 12:159–175.
McCune RC, Syrbu SI, Vasef MA (2006) Expression profiling of transcription factors Pax-5, Oct-1, Oct-2, BOB.1, and PU.1 in Hodgkin’s and non-Hodgkin’s lymphomas: a comparative study using high throughput tissue microarrays. Mod Pathol 19:1010–1018.
Pangalis GA, Angelopoulou MK, Vassilakopoulos TP (1999) B-chronic lymphocytic leukemia, small lymphocytic lymphoma, and lymphoplasmacytic lymphoma, including Waldenström’s macroglobulinemia: a clinical, morphologic, and biologic spectrum of similar disorders. Semin Hematol 36:104–114.
Berger F, Muller-Hermelink HK, Isaacson PG et al (2001) Lymphoplasmacytic lymphoma / Waldenstrom macroglob-ulinemia. In: Jaffe ES, Harris NL, Stein H, Vardiman JW (eds) Pathology and Genetics of Tumours of Hematopoietic and Lymphoid Tissues. World Health Organization Classification of Tumours. IARC Press, Lyon, pp 132–134.
O’Connell FP, Pinkus JL, Pinkus GS (2004) CD138 (syndecan-1), a plasma cell marker immunohistochemical profile in hematopoietic and nonhematopoietic neoplasms. Am J Clin Pathol 121:254–263.
Banks PM, Chan J, Cleary ML et al (1992) Mantle cell lymphoma. A proposal for unification of morphologic, immunologic, and molecular data. Am J Surg Pathol 16:637–640.
Rassidakis GZ, Tani E, Svedmyr E et al (1999) Diagnosis and subclassification of follicle center and mantle cell lymphomas on fine-needle aspirates: A cytologic and immunocytochemical approach based on the Revised European-American Lymphoma (REAL) classification. Cancer 87:216–223.
Nathwani BN, Pirls MA, Harris NL et al (2001) Follicular lymphoma. In: Jaffe ES, Harris NL, Stein H, Vardiman JW (eds) Pathology and Genetics of Tumours of Hematopoietic and Lymphoid Tissues. World Health Organization Classification of Tumours. IARC Press, Lyon, pp 162–167.
Gong Y, Caraway N, Gu J et al (2003) Evaluation of interphase fluorescence in situ hybridization for the t(14;18)(q32;q21) translocation in the diagnosis of follicular lymphoma on fine-needle aspirates: a comparison with flow cytometry immunophenotyping. Cancer 99:385–393.
Sun W, Caraway NP, Zhang HZ et al (2004) Grading follicular lymphoma on fine needle aspiration specimens. Comparison with proliferative index by DNA image analysis and Ki-67 labeling index. Acta Cytol 48:119–126.
Isaacson PG, Harris NL, Nathwani BN et al (2001) Nodal marginal zone B-cell lymphoma. In: Jaffe ES, Harris NL, Stein H, Vardiman JW (eds) Pathology and Genetics of Tumours of Hematopoietic and Lymphoid Tissues. World Health Organization Classification of Tumours. IARC Press, Lyon, p 161.
Campo E, Miquel R, Krenacs L et al (1999) Primary nodal marginal zone lymphomas of splenic and MALT type. Am J Surg Pathol 23:59–68.
Isaacson PG, Du MQ (2004) MALT lymphoma: from morphology to molecules. Nat Rev Cancer 4:644–653.
Pinkus GS, O’Hara CJ, Said JW (1990). Peripheral/postthymic T-cell lymphomas: a spectrum of disease. Clinical, pathologic, and immunologic features of 78 cases. Cancer 65:971–998.
Mayall F, Darlington A, Harrison B (2003) Fine needle aspiration cytology in the diagnosis of uncommon types of lymphoma. J Clin Pathol 56:821–825.
Manosca F, Ariga R, Bengana C et al (2004) Fine-needle aspiration of subcutaneous panniculitis-like T-cell lymphoma. Diagn Cytopathol 31:338–339.
Delsol G, Ralfkiaer E, Stein H et al (2001) Anaplastic large cell lymphoma. In: Jaffe ES, Harris NL, Stein H, Vardiman JW (eds) Pathology and Genetics of Tumours of Hematopoietic and Lymphoid Tissues. World Health Organization Classification of Tumours. IARC Press, Lyon, pp 230–235.
Ng WK, Ip P, Choy C, Collins RJ (2003) Cytologic and immunocytochemical findings of anaplastic large cell lymphoma: analysis of ten fine-needle aspiration specimens over a 9-year period. Cancer 99:33–43.
Rapkiewicz A, Wen H, Sen F, Das K (2007) Cytomorphologic examination of anaplastic large cell lymphoma by fine-needle aspiration cytology. Cancer 111:499–507.
McCluggage WG, Anderson N, Herron B, Caughley L (1996) Fine needle aspiration cytology, histology and immunohistochemistry of anaplastic large cell Ki-1-positive lymphoma. A report of three cases. Acta Cytol 40:779–785.
Kim SE, Kim SH, Lim BJ et al (2004) Fine needle aspiration cytology of small cell variant of anaplastic large cell lymphoma. A case report. Acta Cytol 48:254–258.
Bernard A, Boumsell L, Patte C, Lemerie J (1986) Leukemia versus lymphoma in children: a worthless question? Med Pediatr Oncol 14:148–157.
Soslow RA, Baergen RN, Warnke RA (1999) B-lineage lymphoblastic lymphoma is a clinicopathologic entity distinct from other histologically similar aggressive lymphomas with blastic morphology. Cancer 85:2648–2654.
Sheibani K, Nathwani BN, Winberg CD et al (1987) Antigenically defined subgroups of lymphoblastic lymphoma. Relationship to clinical presentation and biologic behavior. Cancer 60:183–190.
Hughes JH, Caraway NP, Katz RL (1998) Blastic variant of mantle-cell lymphoma: cytomorphologic, immunocyto-chemical, and molecular genetic features of tissue obtained by fine-needle aspiration biopsy. Diagn Cytopathol 19:59–62.
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 2009 Springer-Verlag Italia
About this chapter
Cite this chapter
Gherardi, G. (2009). Lymph Nodes: Diagnosis of Malignant Lymphoma. In: Fine-Needle Biopsy of Superficial and Deep Masses. Springer, Milano. https://doi.org/10.1007/978-88-470-1433-6_4
Download citation
DOI: https://doi.org/10.1007/978-88-470-1433-6_4
Publisher Name: Springer, Milano
Print ISBN: 978-88-470-1432-9
Online ISBN: 978-88-470-1433-6
eBook Packages: MedicineMedicine (R0)