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Infectious Diseases and Nanomedicine II pp 15–23Cite as

Immunological Markers that Correlate with Protection Immunity Against Tularemia Infection

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 808))

Abstract

An efficient immune response to tularemia is dependent on a strong cell-mediated component. We tried to identify markers of cellular immune responses that indicate a vaccine efficacy against tularemia. BALB/c mice were immunized with mutant F. tularensis 15∆23A and/or F. tularensis 15 NIIEG strains and then were challenged i.n. with F. tularensis Schu. We compared the influence of F. tularensis antigens (tularinum) in vitro on production of IL-1, IL-5, IL-6, IL-17, IFN-γ, and TNF-α by splenocytes obtained from intact mice and mice immunized with mutant F. tularensis 15∆23A and/or F. tularensis 15 NIIEG strains. We also compared expression of CD28, CD154, TLR-2, and CD69 markers on CD4 and CD8 T-cells after activation with tularinum in vitro. We found that tularinum-induced CD4+ T-cells increased TNF-α and IFN-γ synthesis and expression of CD69 only in group mice with high degree of post immunization protection against F. tularensis Schu challenge. Estimation of CD69 expression on CD3+CD4+ cells and IFN-γ, TNF-α synthesis by CD4+ T-lymphocytes could be useful for determination protect ability of antitularemia immunity.

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Authors and Affiliations

  1. State Research Center for Applied Microbiology and Biotechnology, Moscow, Obolensk, Russia, 142279

    Victoria V. Firstova, Alexander N. Mokrievich, Vitalii M. Pavlov, Aleksey A. Gorbatov, Tatiana I. Kombarova, Sergey F. Biketov & Ivan A. Dyatlov

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  1. Victoria V. Firstova
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  2. Alexander N. Mokrievich
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  3. Vitalii M. Pavlov
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  4. Aleksey A. Gorbatov
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  5. Tatiana I. Kombarova
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  6. Sergey F. Biketov
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  7. Ivan A. Dyatlov
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Correspondence to Victoria V. Firstova .

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Editors and Affiliations

  1. Central Department of Chemistry, Tribhuvan University, Kathmandu, Nepal

    Rameshwar Adhikari

  2. Department of Microbiology, Tribhuvan University, Kathmandu, Nepal

    Santosh Thapa

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Firstova, V.V. et al. (2014). Immunological Markers that Correlate with Protection Immunity Against Tularemia Infection. In: Adhikari, R., Thapa, S. (eds) Infectious Diseases and Nanomedicine II. Advances in Experimental Medicine and Biology, vol 808. Springer, New Delhi. https://doi.org/10.1007/978-81-322-1774-9_2

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