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Glycoside Hydrolases for Extraction and Modification of Polyphenolic Antioxidants

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Advances in Enzyme Biotechnology

Abstract

Antioxidants are important molecules that are widely used by humans, both as dietary supplements and as additives to different types of products. In this chapter, we review how flavonoids, a class of polyphenolic antioxidants that are often found in glycosylated forms in many natural resources, can be extracted and modified using glycoside hydrolases (GHs). Glycosylation is a fundamental enzymatic process in nature, affecting function of many types of molecules (glycans, proteins, lipids as well as other organic molecules such as the flavonoids). Possibilities to control glycosylation thus mean possibilities to control or modify the function of the molecule. For the flavonoids, glycosylation affect both the antioxidative power and solubility. In this chapter we overview results on in vitro deglycosylation and glycosylation of flavonoids by selected GHs. For optimal enzymatic performance, desired features include a correct specificity for the target, combined with high stability. Poor specificity towards a specific substituent is thus a major drawback for enzymes in particular applications. Efforts to develop the enzymes as conversion tools are reviewed.

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Acknowledgements 

The authors wish to thank Formas 2009-1527 (SuReTech), the European project AMYLOMICS and the Antidiabetic Food Centre, a VINNOVA VINN Excellence Centre at Lund University.

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Authors and Affiliations

  1. Biotechnology, Department of Chemistry, Lund University, 124, SE-22100, Lund, Sweden

    Kazi Zubaida Gulshan Ara, Samiullah Khan, Tejas S. Kulkarni, Tania Pozzo & Eva Nordberg Karlsson

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  1. Kazi Zubaida Gulshan Ara
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  2. Samiullah Khan
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  3. Tejas S. Kulkarni
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  4. Tania Pozzo
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  5. Eva Nordberg Karlsson
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Correspondence to Eva Nordberg Karlsson .

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Editors and Affiliations

  1. Department of Microbiology, Maharshi Dayanand University, Rohtak, Haryana, India

    Pratyoosh Shukla

  2. Department of Biochemistry,, Rhodes University, Grahamstown, South Africa

    Brett I. Pletschke

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Ara, K.Z.G., Khan, S., Kulkarni, T.S., Pozzo, T., Karlsson, E.N. (2013). Glycoside Hydrolases for Extraction and Modification of Polyphenolic Antioxidants. In: Shukla, P., Pletschke, B. (eds) Advances in Enzyme Biotechnology. Springer, New Delhi. https://doi.org/10.1007/978-81-322-1094-8_2

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