Abstract
Epithelial branching morphogenesis is important to form many organs. Embryonic salivary glands provide an excellent model for clarifying the mechanisms of this phenomenon. As clefts form, epithelial cell–cell adhesions are converted to cell–matrix adhesions. Nevertheless, the mechanism of cleft formation is not well understood. Here, we describe a set of approaches being used to identify and characterize molecules necessary for branching morphogenesis. A combination of laser microdissection with T7-SAGE has been established as a gene discovery method for identifying candidate molecules that may be essential for early organ morphogenesis. Progress in understanding the mechanisms of salivary branching morphogenesis will provide novel approaches to future tissue engineering or regeneration of damaged salivary glands.
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Acknowledgment
This research was supported by the Intramural Research Program of the NIDCR, NIH, and Grant-in Aid for Scientific Research (B) in Japan Society for the Promotion of Science. We thank Yukinori Endo and Jill Harunaga for comments on this manuscript.
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Sakai, T., Onodera, T., Yamada, K.M. (2010). Cleft formation and branching morphogenesis of salivary gland: exploration of new functional genes. In: Sasano, T., Suzuki, O. (eds) Interface Oral Health Science 2009. Springer, Tokyo. https://doi.org/10.1007/978-4-431-99644-6_2
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DOI: https://doi.org/10.1007/978-4-431-99644-6_2
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-99643-9
Online ISBN: 978-4-431-99644-6
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