Abstract
To allow observation of cellular ultrastructure in foraminifers (or other meiofauna) preserved in their life position within sediments, modifications of the Fluorescently Labeled Embedded Core (FLEC) method are described. Such an approach will allow determinations of both fine-scale (sub-millimeter scale) distributional data and cell biological data on the same specimen.
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Acknowledgements
Support for this research was provided by the US NSF grant OCE-0926421 to J.M.B. and V.P. Edgcomb. We thank Sam Bowser for insightful discussions and helpful comments on an earlier manuscript version. J.M.B. also thanks JAMSTEC and Tohoku University for financial support to allow her contributions to the Field Workshop on Living Foraminifera in Japan in 2012.
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Bernhard, J.M., Richardson, E.A. (2014). FLEC-TEM: Using Microscopy to Correlate Ultrastructure with Life Position of Infaunal Foraminifera. In: Kitazato, H., M. Bernhard, J. (eds) Approaches to Study Living Foraminifera. Environmental Science and Engineering(). Springer, Tokyo. https://doi.org/10.1007/978-4-431-54388-6_7
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