Abstract
We found that self-organized honeycomb-patterned porous polymer films (honeycomb films) prepared by casting polymer dissolved in a water-immiscible solvent under high humidity. We demonstrated that the microtopography of the honeycomb film strongly affected human mesenchymal stem cells (hMSCs) and periodontal ligament (PDL) cells, which were the important cell sources for tissue engineering. hMSCs on the honeycomb films having small-sized (1.5 μm) pores induced a dramatic stem cell spheroid formation. PDL cells on the honeycomb films (pore sizes of 10 μm) formed increasingly elongated cell shape to trap in their pores. The honeycomb films, which controlled cellular morphology by changing only the geometric cues without the inducing media, can be applied as functional biomaterials for the regenerative therapy.
Key words
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Hubbell JA (1995) Biomaterials in tissue engineering. Nat Biotechnol 13:565–576
Langer R, Vacanti J (1993) Tissue engineering. Science 260:920–926
Daley WP, Peters SB, Larsen M (2008) Extracellular matrix dynamics in development and regenerative medicine. J Cell Sci 121:255–264
Carter SB (1965) Principles of cell motility: the direction of cell movement and cancer invasion. Nature 208:1183–1187
Engler AJ, Sen S, Sweeney HL, Discher DE (2006) Matrix elasticity directs stem cell lineage specification. Cell 126:677–689
Weiss P (1985) Cell contact. Int Rev Cytol 7:391–423
Dalby MJ, Gadegaard N, Tare R, Andar A, Riehle MO, Herzyk P, Wilkinso CDW, Oreffo ROC (2007) The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder. Nat Mater 6:997–1003
Maruyama N, Koito T, Sawadaishi T, Karthaus O, Ijiro K, Nishi N, Tokura S, Nishimura S, Shimomura M (1998) Mesoscopic pattern formation of nanostructured polymer assemblies. Supramol Sci 5:331–336
Karthaus O, Maruyama N, Cieren X, Shimomura M, Hasegawa H, Hashimoto T (2000) Water-assisted formation of micrometer-size honeycomb patterns of polymers. Langmuir 16:6071–6076
Yabu H, Hirai Y, Shimomura M (2006) Electroless plating of honeycomb and pincushion polymer films prepared by self-organization. Langmuir 22:9760–9764
Fukuhira Y, Kitazono E, Hayashi T, Kaneko H, Tanaka M, Shimomura M, Sumi Y (2006) Biodegradable honeycomb-patterned film composed of poly(lactic acid) and dioleoylphosphatidylethanolamine. Biomaterials 27:1797–1802
McBeath R, Pirone DM, Nelson CM, Bhadriraju K, Chen CC (2004) Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment. Dev Cell 6:483–495
Gomez Flores M, Hasegawa M, Yamato M, Takagi R, Okano T, Ishikawa I (2008) Cementum–periodontal ligament complex regeneration using the cell sheet technique. J Periodontal Res 43:364–371
Amemiya T, Adachi K, Nishigaki M, Yamamoto T, Kanamura N (2008) Experiences of preclinical use of periodontal ligament-derived cell sheet cultured on human amniotic membrane. J Oral Tissue Eng 6:106–112
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer
About this paper
Cite this paper
Kawano, T., Iwama, N., Ishihata, H., Shimauchi, H., Shimomura, M. (2012). Preparation and Biomedical Application of Self-Organized Honeycomb-Patterned Polymer Films. In: Sasaki, K., Suzuki, O., Takahashi, N. (eds) Interface Oral Health Science 2011. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54070-0_4
Download citation
DOI: https://doi.org/10.1007/978-4-431-54070-0_4
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-54069-4
Online ISBN: 978-4-431-54070-0
eBook Packages: MedicineMedicine (R0)