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Chembiomolecular Science pp 127–138Cite as

High-Throughput Screening for Small Molecule Modulators of FGFR2-IIIb Pre-mRNA Splicing

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Abstract

Pre-mRNA splicing is the process in which a ribonucleoprotein complex called the spliceosome removes introns from a primary transcript and ligates its exons together [1]. The spliced exons produce an mRNA containing the mature protein coding sequence. The splicing process is remarkably consistent in its choice of splice sites, and these choices can be regulated to allow different mRNA sequences to arise from the same pre-mRNA transcript, in a process called alternative splicing. In this way, genes often yield multiple mRNAs and encoded proteins to increase diversity of eukaryotic proteomes. Alternative splicing patterns often show developmental and tissue-specific expression. Misregulation of splicing is seen in human disease and can be a direct cause of pathology [2]. Splicing regulatory mechanisms thus present a promising target for therapeutic intervention.

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Authors and Affiliations

  1. Molecular Biology Institute, University of California, Paul D. Boyer Hall, 611 Charles E. Young Drive East, 951570, Los Angeles, CA, 90095-1570, USA

    Erik S. Anderson

  2. The David Geffen School of Medicine, University of California, 23-385 Center for the Health Sciences, 957041, Los Angeles, CA, 90095-7041, USA

    Erik S. Anderson

  3. Department of Biochemistry, West Virginia University, 3080I HSN, Robert C. Byrd Health Sciences Center, 1 Medical Center Drive, 9142, Morgantown, WV, 26506, USA

    Peter Stoilov

  4. Molecular Screening Shared Resource, University of California, 2145 California NanoSystems Institute (CNSI), 570 Westwood Plaza, Building 114/MC-722710, Los Angeles, CA, 90095, USA

    Robert Damoiseaux

  5. Howard Hughes Medical Institute, University of California, 5-748 MacDonald Research Laboratories, 675 Charles E. Young Drive South, Los Angeles, CA, 90095-1662, USA

    Douglas L. Black

  6. Department of Microbiology, Immunology and Molecular Genetics, University of California, 609 Charles E. Young Dr. East, Los Angeles, CA, 90095-1489, USA

    Douglas L. Black

Authors
  1. Erik S. Anderson
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  2. Peter Stoilov
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  3. Robert Damoiseaux
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  4. Douglas L. Black
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Corresponding author

Correspondence to Douglas L. Black .

Editor information

Editors and Affiliations

  1. Institute of Microbial Chemistry, Kamioosaki 3-14-23, Tokyo, 141-0021, Japan

    Masakatsu Shibasaki

  2. The University of Tokyo, Department of Pharmacology, Graduate School of Medicine, Hongo 7-3-1, Bunkyo-ku, 113-0033, Japan

    Masamitsu Iino

  3. RIKEN Advanced Science Institute, Antibiotics Laboratory, Chemical Biology Core Facility, 2-1, Hirosawa, Wako, 351-0198, Saitama, Japan

    Hiroyuki Osada

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© 2012 Springer

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Anderson, E.S., Stoilov, P., Damoiseaux, R., Black, D.L. (2012). High-Throughput Screening for Small Molecule Modulators of FGFR2-IIIb Pre-mRNA Splicing. In: Shibasaki, M., Iino, M., Osada, H. (eds) Chembiomolecular Science. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54038-0_12

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