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Microarrays in Inflammation pp 95–104Cite as

Amplification of cDNA from single or rare cells by global PCR (exponential amplification)

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Part of the book series: Progress in Inflammation Research ((PIR))

Abstract

In this chapter we provide a brief overview of protocols used to amplify mRNA isolated from single cells. We focus on PCR-based protocols and summarize our experiences in the use of amplified single cell cDNA for microarray hybridization.

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Author information

Authors and Affiliations

  1. Division of Oncogenomics, Department of Pathology, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053, Regensburg, Germany

    Christoph A. Klein & Claudia H. Hartmann

Authors
  1. Christoph A. Klein
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  2. Claudia H. Hartmann
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Editor information

Editors and Affiliations

  1. Research & Development, Miltenyi Biotec GmbH, Friedrich-Ebert-Straße 68, 51429 Bergisch, Gladbach, Germany

    Andreas Bosio

  2. Miltenyi Biotec GmbH, Friedrich-Ebert-Straße 68, 51429 Bergisch, Gladbach, Germany

    Bernhard Gerstmayer

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© 2008 Birkhäuser Verlag Basel/Switzerland

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Klein, C.A., Hartmann, C.H. (2008). Amplification of cDNA from single or rare cells by global PCR (exponential amplification). In: Bosio, A., Gerstmayer, B. (eds) Microarrays in Inflammation. Progress in Inflammation Research. Birkhäuser Basel. https://doi.org/10.1007/978-3-7643-8334-3_9

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