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Differential display and protein quantification

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Plant Systems Biology

Part of the book series: Experientia Supplementum ((EXS,volume 97))

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Abstract

High-throughput quantitation of proteins is of essential importance for all systems biology approaches and provides complementary information on steady-state gene expression and perturbation-induced systems responses. This information is necessary because it is, e.g., difficult to predict protein concentrations from the level of mRNAs, since regulatory processes at the posttranscriptional level adjust protein concentrations to prevailing conditions. Despite its importance, quantitative proteomics is still a challenging task because of the high dynamic range of protein concentrations in the cell and the variation in the physical properties of proteins. In this chapter we review the current status of, and options for, protein quantification in high-throughput experiments and discuss the suitability and limitations of different existing methods.

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Authors and Affiliations

  1. Functional Genomics Center Zürich, Winterthurerstr. 190, 8057, Zürich, Switzerland

    Erich Brunner, Bertran Gerrits, Mike Scott & Bernd Roschitzki

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  1. Erich Brunner
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  2. Bertran Gerrits
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  3. Mike Scott
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  4. Bernd Roschitzki
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Editors and Affiliations

  1. Institute of Plant Sciences, Swiss Federal Institute of Technology, ETH Zentrum, LFW E, 8092, Zürich, Switzerland

    Sacha Baginsky

  2. MPI for Molecular Plant Physiology, Am Mühlenberg 1, 14476, Golm, Germany

    Alisdair R. Fernie

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Brunner, E., Gerrits, B., Scott, M., Roschitzki, B. (2007). Differential display and protein quantification. In: Baginsky, S., Fernie, A.R. (eds) Plant Systems Biology. Experientia Supplementum, vol 97. Birkhäuser Basel. https://doi.org/10.1007/978-3-7643-7439-6_6

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